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  1. The present study describes a protocol to generate heterogenous populations of neurotransmitter-producing neurons from human mesenchymal stem cells (MSCs). MSCs are bone marrow (BM)-derived cells which undergo...

    Authors: Steven J. Greco, Chunyi Zhou, Jiang-Hong Ye and Pranela Rameshwar
    Citation: Biological Procedures Online 2008 10:101000090
  2. The early chick embryo contains subpopulations of cells that express lineage-specific transcription factors. We have developed protocols to examine the role of these cells during development that involve label...

    Authors: Jacquelyn Gerhart, Christine Neely, Jessica Pfautz and Mindy George-Weinstein
    Citation: Biological Procedures Online 2008 10:101000074
  3. Gene families are widely used in comparative genomics, molecular evolution, and in systematics. However, they are constructed in different manners, their data analyzed and interpreted differently, with differe...

    Authors: David A. Liberles and Katharina Dittmar
    Citation: Biological Procedures Online 2008 10:101066
  4. DNA methyltransferase 1 (DNMT1) is the enzyme responsible for the maintenance of DNA methylation patterns during cell division. DNMT1 expression is tightly regulated within the cell cycle. Our previous study s...

    Authors: Alexander Unterberger, Jérôme Torrisani and Moshe Szyf
    Citation: Biological Procedures Online 2008 10:101047
  5. Acetyl phosphate (acetyl-P) serves critical roles in coenzyme A recycling and ATP synthesis. It is the intermediate of the Pta-AckA pathway that inter-converts acetyl-coenzyme A and acetate. Acetyl-P also can ...

    Authors: David H. Keating, Ana Shulla, Adam H. Klein and Alan J. Wolfe
    Citation: Biological Procedures Online 2008 10:101036
  6. Calcitriol (1α, 25-dihydroxyvitamin D3) induces the expression of CD14 in mononuclear phagocytes. The mechanisms accounting for this have been unclear since the promoter of CD14 does not contain a canonical vitam...

    Authors: Alireza Moeenrezakhanlou, Devki Nandan and Neil E. Reiner
    Citation: Biological Procedures Online 2008 10:101029
  7. Understanding mechanisms of gene regulation has broad therapeutic implications for human disease. Here we describe a novel method for generating human cell lines that serve as reporters of transcriptional acti...

    Authors: Sandra L. Fernandez, David W. Russell and Peter J. Hurlin
    Citation: Biological Procedures Online 2007 9:91084
  8. B cell subpopulations in the spleen have been extensively characterized phenotypically; however, biochemical properties of these cell populations following B cell antigen receptor engagement have not been full...

    Authors: Pierre Antony, Kristen Hoek, Bhaskarjyoti Sarmah and Wasif N. Khan
    Citation: Biological Procedures Online 2007 9:91073
  9. Recent advances in redox proteomics have provided significant insight into the role of oxidative modifications in cellular signalling and metabolism. At present, these techniques rely heavily on Western blots ...

    Authors: H. Fai Poon, Laila Abdullah, Jon Reed, Sarah M. Doore, Cyndi Laird, Venkat Mathura, Michael Mullan and Fiona Crawford
    Citation: Biological Procedures Online 2007 9:91065
  10. In adults, hematopoiesis occurs in bone marrow (BM) through a complex process with differentiation of hematopoietic stem cells (HSCs) to immune and blood cells. Human HSCs and their progenitors express CD34. M...

    Authors: Nitixa Patel, Marianne Castillo and Pranela Rameshwar
    Citation: Biological Procedures Online 2007 9:91056
  11. Teleost gut associated lymphoid tissue (GALT) consists of leucocyte populations located both intraepithelially and in the lamina propria with no structural organization. The present study aims to assess differ...

    Authors: Irene Salinas, José Meseguer and Maria Ángeles Esteban
    Citation: Biological Procedures Online 2007 9:91043
  12. We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cass...

    Authors: Seok Hwee Koo, Tan Ching Ong, Kok Ting Chong, Caroline Guat Lay Lee, Fook Tim Chew and Edmund Jon Deoon Lee
    Citation: Biological Procedures Online 2007 9:91027
  13. We developed a rapid mutagenesis method based on a modification of the QuikChange® system (Stratagene) to systemically replace endogenous gene sequences with a unique similar size sequence tag. The modificatio...

    Authors: Qiwen Deng, Wensheng Luo and Michael S. Donnenberg
    Citation: Biological Procedures Online 2007 9:91018
  14. Suppression of gene expression by small interfering RNA (siRNA) has proved to be a gene-specific and cost effective alternative to other gene suppression technologies. Short hairpin RNAs (shRNAs) generated fro...

    Authors: Hailong Wu, Anh Dinh and Yin-Yuan Mo
    Citation: Biological Procedures Online 2007 9:91009
  15. We have developed a system to visualize functionally activated neurons and their projections in the brain. This system utilizes a transgenic mouse, fos-tau-lacZ (FTL), which expresses the marker gene, lacZ, in ne...

    Authors: Mark Murphy, Ursula Greferath and Yvette M. Wilson
    Citation: Biological Procedures Online 2007 9:91001
  16. Microbes frequently live within multicellular, solid surface-attached assemblages termed biofilms. These microbial communities have architectural features that contribute to population heterogeneity and conseq...

    Authors: Joe J. Harrison, Howard Ceri, Jerome Yerly, Carol A. Stremick, Yaoping Hu, Robert Martinuzzi and Raymond J. Turner
    Citation: Biological Procedures Online 2006 8:81194
  17. Quantitative real-time PCR (qPCR) is a commonly used validation tool for confirming gene expression results obtained from microarray analysis; however, microarray and qPCR data often result in disagreement. Th...

    Authors: Jeanine S. Morey, James C. Ryan and Frances M. Van Dolah
    Citation: Biological Procedures Online 2006 8:81175
  18. This paper was originally published in Biological Procedures Online (BPO) on March 23, 2006. It was brought to the attention of the journal and authors that reference 74 was incorrect. The original citation for r...

    Authors: M. B. I. Reaz, M. S. Hussain and F. Mohd-Yasin
    Citation: Biological Procedures Online 2006 8:81163
  19. In this work we describe protocols for the generation of gene deletions and gene replacements using a temperature sensitive plasmid in Escherichia coli O157:H7. This technology requires flanking DNA to be cloned ...

    Authors: James R. Emmerson, David L. Gally and Andrew J. Roe
    Citation: Biological Procedures Online 2006 8:81153
  20. The purpose of this manuscript is to discuss fluorogenic real-time quantitative polymerase chain reaction (qPCR) inhibition and to introduce/define a novel Microsoft Excel-based file system which provides a wa...

    Authors: Jack M. Gallup and Mark R. Ackermann
    Citation: Biological Procedures Online 2006 8:81087
  21. Live cell fluorescence microscopy using fluorescent protein tags derived from jellyfish and coral species has been a successful tool to image proteins and dynamics in many species. Multi-colored aequorea fluoresc...

    Authors: Jianrun Xia, Song Hon H. Kim, Susan Macmillan and Ray Truant
    Citation: Biological Procedures Online 2006 8:81063
  22. The application of naked DNA containing type I interferon (IFN) transgenes is a promising potential therapeutic approach for controlling chronic viral infections. Herein, we detail the application of this appr...

    Authors: Bobbie Ann Austin, Cassandra M. James, Peter Härle and Daniel J. J. Carr
    Citation: Biological Procedures Online 2006 8:81055
  23. The Chromatin Immunoprecipiation (ChIP) provides a powerful technique for identifying the in vivo association of transcription factors with regulatory elements. However, obtaining meaningful information for promo...

    Authors: Rebecca L. Dryer and Lori R. Covey
    Citation: Biological Procedures Online 2006 8:81044
  24. Microtubule inhibitors such as Vinblastine and Paclitaxel are chemotherapy agents that activate the mitotic spindle checkpoint, arresting cells in mitosis and leading to cell death. The pathways that connect m...

    Authors: Hiroshi Y. Yamada and Gary J. Gorbsky
    Citation: Biological Procedures Online 2006 8:81036
  25. Pancreatic β-cell apoptosis is known to participate in the β-cell destruction process that occurs in diabetes. It has been described that high glucose level induces a hyperfunctional status which could provoke...

    Authors: Jose M. Mellado-Gil and Manuel Aguilar-Diosdado
    Citation: Biological Procedures Online 2005 7:710162
  26. Efficient protein folding and quality control are essential for unperturbed cell viability. Defects in these processes may lead to production of aberrant polypeptides that are either degraded leading to “loss-...

    Authors: Tatiana Soldà, Silvia Olivari and Maurizio Molinari
    Citation: Biological Procedures Online 2005 7:710136
  27. We propose two-dimensional gel electrophoresis (2-DE) and mass spectrometry to define the protein components of regulons and stimulons in bacteria, including those organisms where genome sequencing is still in...

    Authors: Sergio Encarnación, Magdalena Hernández, Gabriel Martínez-Batallar, Sandra Contreras, María del Carmen Vargas and Jaime Mora
    Citation: Biological Procedures Online 2005 7:710117
  28. Most behavioral experiments within circadian research are based on the analysis of locomotor activity. This paper introduces scientists to chronobiology by explaining the basic terminology used within the fiel...

    Authors: Corinne Jud, Isabelle Schmutz, Gabriele Hampp, Henrik Oster and Urs Albrecht
    Citation: Biological Procedures Online 2005 7:710101
  29. We have developed a simple and effective method (Lig-PCR) for monitoring ligation reactions using PCR and primers that are common to many cloning vectors. Ligation mixtures can directly be used as templates an...

    Authors: Prafulla K. Chandra and Stephen K. Wikel
    Citation: Biological Procedures Online 2005 7:710093
  30. We describe a new approach for reliably isolating one-step real-time quantitative RT-PCR-quality RNA from laser captured cells retrieved from frozen sections previously subjected to immunofluorescent immunohis...

    Authors: Jack M. Gallup, Kenji Kawashima, Ginger Lucero and Mark R. Ackermann
    Citation: Biological Procedures Online 2005 7:710070
  31. Mature osteoclasts, multinucleated giant cells responsible for bone resorption, are terminally differentiated cells with a short life span. Recently, we have demonstrated that osteoclast apoptosis is regulated...

    Authors: Toru Akiyama, Tsuyoshi Miyazaki, Phillippe Bouillet, Kozo Nakamura, Andreas Strasser and Sakae Tanaka
    Citation: Biological Procedures Online 2005 7:710048
  32. Angiogenesis is a complex process involving an ECM and vascular endothelial cells (EC), and is regulated by various angiogenic factors including VEGF. The ability to form a capillary/tube-like network is a spe...

    Authors: Motomu Watanabe, Yayoi Fujioka-Kaneko, Hisayuki Kobayashi, Mamoru Kiniwa, Michihiko Kuwano and Yuji Basaki
    Citation: Biological Procedures Online 2005 7:710041
  33. We describe an improved, efficient and reliable method for the vapour-phase silanization of multi-barreled, ion-selective microelectrodes of which the silanized barrel(s) are to be filled with neutral liquid i...

    Authors: Jason S. T. Deveau, Michael I. Lindinger and Bernard Grodzinski
    Citation: Biological Procedures Online 2005 7:710031
  34. Cell culturing, and the requisite storage of cell lines at ultra-low temperatures, is used in most laboratories studying or using eukaryotic proteomics, genomics, microarray, and RNA technologies. In this stud...

    Authors: Joe Corsini and Ethan Mann
    Citation: Biological Procedures Online 2005 7:710026
  35. To assess the role of sortilin in the sorting and trafficking of sphingolipid activator proteins (SAPs) the function of sortilin was abolished by a dominant-negative mutant and by the use of RNAi. Mutant sorti...

    Authors: Stephane Lefrancois, Maryssa Canuel, Jibin Zeng and Carlos R. Morales
    Citation: Biological Procedures Online 2005 7:710017
  36. To date, breast cancer (BC) research is mainly studied with cell lines. These cells were passaged multiple times, acquiring phenotypes, additional mutations and epigenetic changes. These changes make the passa...

    Authors: Kelly E. Corcoran, Prem S. Patel and Pranela Rameshwar
    Citation: Biological Procedures Online 2005 7:710008

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