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  5. Comparative genomics has become a real tantalizing challenge in the postgenomic era. This fact has been mostly magnified by the plethora of new genomes becoming available in a daily bases. The overwhelming lis...

    Authors: Jennifer Commins, Christina Toft and Mario A Fares
    Citation: Biological Procedures Online 2009 11:52
    Content type: Review Published on:

  6. The dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with enzyme-fre...

    Authors: Boon C Heng, Catherine M Cowan and Shubhayu Basu
    Citation: Biological Procedures Online 2009 11:161
    Content type: Research Published on:

  7. Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for...

    Authors: Alina Rodrƭguez-Mallon, Yamilet CƔrdenas, Juana Marƭa Lugo, AymƩ Oliva, Antonio Morales and Mario Pablo Estrada
    Citation: Biological Procedures Online 2009 11:79
    Content type: Methodology Published on:

  8. As many as 50% of adults with type I (T1) diabetes exhibit bone loss and are at increased risk for fractures. Therapeutic development to prevent bone loss and/or restore lost bone in T1 diabetic patients requi...

    Authors: Katherine Motyl and Laura R McCabe
    Citation: Biological Procedures Online 2009 11:296
    Content type: Research Published on:

  9. Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the complex organization of the mammalian testicular tissue, var...

    Authors: Rosana RodrĆ­guez-Casuriaga, Adriana Geisinger, Beatriz LĆ³pez-Carro, Valentina Porro, Rodolfo Wettstein and Gustavo A Folle
    Citation: Biological Procedures Online 2009 11:184
    Content type: Methodology Published on:

  10. The present study describes a protocol to generate heterogenous populations of neurotransmitter-producing neurons from human mesenchymal stem cells (MSCs). MSCs are bone marrow (BM)-derived cells which undergo...

    Authors: Steven J. Greco, Chunyi Zhou, Jiang-Hong Ye and Pranela Rameshwar
    Citation: Biological Procedures Online 2008 10:101000090
    Content type: OriginalPaper Published on:

  11. In this paper, we describe a simple, rapid, specific, sensitive, and reliable method, the FICP method (Fluorescence Immunoassay for Cellular Protein detection) which is readily applicable to the detection of p...

    Authors: Carole Lavigne, ArmĆØle de GuignĆ©e and Alain R. Thierry
    Citation: Biological Procedures Online 2008 10:101000083
    Content type: OriginalPaper Published on:

  12. The early chick embryo contains subpopulations of cells that express lineage-specific transcription factors. We have developed protocols to examine the role of these cells during development that involve label...

    Authors: Jacquelyn Gerhart, Christine Neely, Jessica Pfautz and Mindy George-Weinstein
    Citation: Biological Procedures Online 2008 10:101000074
    Content type: OriginalPaper Published on:

  13. Gene families are widely used in comparative genomics, molecular evolution, and in systematics. However, they are constructed in different manners, their data analyzed and interpreted differently, with differe...

    Authors: David A. Liberles and Katharina Dittmar
    Citation: Biological Procedures Online 2008 10:101066
    Content type: OriginalPaper Published on:

  15. DNA methyltransferase 1 (DNMT1) is the enzyme responsible for the maintenance of DNA methylation patterns during cell division. DNMT1 expression is tightly regulated within the cell cycle. Our previous study s...

    Authors: Alexander Unterberger, JƩrƓme Torrisani and Moshe Szyf
    Citation: Biological Procedures Online 2008 10:101047
    Content type: OriginalPaper Published on:

  16. Acetyl phosphate (acetyl-P) serves critical roles in coenzyme A recycling and ATP synthesis. It is the intermediate of the Pta-AckA pathway that inter-converts acetyl-coenzyme A and acetate. Acetyl-P also can ...

    Authors: David H. Keating, Ana Shulla, Adam H. Klein and Alan J. Wolfe
    Citation: Biological Procedures Online 2008 10:101036
    Content type: OriginalPaper Published on:

  17. Calcitriol (1Ī±, 25-dihydroxyvitamin D3) induces the expression of CD14 in mononuclear phagocytes. The mechanisms accounting for this have been unclear since the promoter of CD14 does not contain a canonical vitam...

    Authors: Alireza Moeenrezakhanlou, Devki Nandan and Neil E. Reiner
    Citation: Biological Procedures Online 2008 10:101029
    Content type: OriginalPaper Published on:

  21. Understanding mechanisms of gene regulation has broad therapeutic implications for human disease. Here we describe a novel method for generating human cell lines that serve as reporters of transcriptional acti...

    Authors: Sandra L. Fernandez, David W. Russell and Peter J. Hurlin
    Citation: Biological Procedures Online 2007 9:91084
    Content type: OriginalPaper Published on:

  22. B cell subpopulations in the spleen have been extensively characterized phenotypically; however, biochemical properties of these cell populations following B cell antigen receptor engagement have not been full...

    Authors: Pierre Antony, Kristen Hoek, Bhaskarjyoti Sarmah and Wasif N. Khan
    Citation: Biological Procedures Online 2007 9:91073
    Content type: OriginalPaper Published on:

  23. Recent advances in redox proteomics have provided significant insight into the role of oxidative modifications in cellular signalling and metabolism. At present, these techniques rely heavily on Western blots ...

    Authors: H. Fai Poon, Laila Abdullah, Jon Reed, Sarah M. Doore, Cyndi Laird, Venkat Mathura, Michael Mullan and Fiona Crawford
    Citation: Biological Procedures Online 2007 9:91065
    Content type: OriginalPaper Published on:

  24. In adults, hematopoiesis occurs in bone marrow (BM) through a complex process with differentiation of hematopoietic stem cells (HSCs) to immune and blood cells. Human HSCs and their progenitors express CD34. M...

    Authors: Nitixa Patel, Marianne Castillo and Pranela Rameshwar
    Citation: Biological Procedures Online 2007 9:91056
    Content type: OriginalPaper Published on:

  25. Teleost gut associated lymphoid tissue (GALT) consists of leucocyte populations located both intraepithelially and in the lamina propria with no structural organization. The present study aims to assess differ...

    Authors: Irene Salinas, JosƩ Meseguer and Maria Ɓngeles Esteban
    Citation: Biological Procedures Online 2007 9:91043
    Content type: OriginalPaper Published on:

  26. We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cass...

    Authors: Seok Hwee Koo, Tan Ching Ong, Kok Ting Chong, Caroline Guat Lay Lee, Fook Tim Chew and Edmund Jon Deoon Lee
    Citation: Biological Procedures Online 2007 9:91027
    Content type: OriginalPaper Published on:

  27. We developed a rapid mutagenesis method based on a modification of the QuikChangeĀ® system (Stratagene) to systemically replace endogenous gene sequences with a unique similar size sequence tag. The modificatio...

    Authors: Qiwen Deng, Wensheng Luo and Michael S. Donnenberg
    Citation: Biological Procedures Online 2007 9:91018
    Content type: OriginalPaper Published on:

  28. Suppression of gene expression by small interfering RNA (siRNA) has proved to be a gene-specific and cost effective alternative to other gene suppression technologies. Short hairpin RNAs (shRNAs) generated fro...

    Authors: Hailong Wu, Anh Dinh and Yin-Yuan Mo
    Citation: Biological Procedures Online 2007 9:91009
    Content type: OriginalPaper Published on:

  29. We have developed a system to visualize functionally activated neurons and their projections in the brain. This system utilizes a transgenic mouse, fos-tau-lacZ (FTL), which expresses the marker gene, lacZ, in ne...

    Authors: Mark Murphy, Ursula Greferath and Yvette M. Wilson
    Citation: Biological Procedures Online 2007 9:91001
    Content type: OriginalPaper Published on:

  30. Microbes frequently live within multicellular, solid surface-attached assemblages termed biofilms. These microbial communities have architectural features that contribute to population heterogeneity and conseq...

    Authors: Joe J. Harrison, Howard Ceri, Jerome Yerly, Carol A. Stremick, Yaoping Hu, Robert Martinuzzi and Raymond J. Turner
    Citation: Biological Procedures Online 2006 8:81194
    Content type: OriginalPaper Published on:

  31. Quantitative real-time PCR (qPCR) is a commonly used validation tool for confirming gene expression results obtained from microarray analysis; however, microarray and qPCR data often result in disagreement. Th...

    Authors: Jeanine S. Morey, James C. Ryan and Frances M. Van Dolah
    Citation: Biological Procedures Online 2006 8:81175
    Content type: OriginalPaper Published on:

  33. This paper was originally published in Biological Procedures Online (BPO) on March 23, 2006. It was brought to the attention of the journal and authors that reference 74 was incorrect. The original citation for r...

    Authors: M. B. I. Reaz, M. S. Hussain and F. Mohd-Yasin
    Citation: Biological Procedures Online 2006 8:81163
    Content type: OriginalPaper Published on:

  34. In this work we describe protocols for the generation of gene deletions and gene replacements using a temperature sensitive plasmid in Escherichia coli O157:H7. This technology requires flanking DNA to be cloned ...

    Authors: James R. Emmerson, David L. Gally and Andrew J. Roe
    Citation: Biological Procedures Online 2006 8:81153
    Content type: OriginalPaper Published on:

  35. The purpose of this manuscript is to discuss fluorogenic real-time quantitative polymerase chain reaction (qPCR) inhibition and to introduce/define a novel Microsoft Excel-based file system which provides a wa...

    Authors: Jack M. Gallup and Mark R. Ackermann
    Citation: Biological Procedures Online 2006 8:81087
    Content type: OriginalPaper Published on:

  38. Live cell fluorescence microscopy using fluorescent protein tags derived from jellyfish and coral species has been a successful tool to image proteins and dynamics in many species. Multi-colored aequorea fluoresc...

    Authors: Jianrun Xia, Song Hon H. Kim, Susan Macmillan and Ray Truant
    Citation: Biological Procedures Online 2006 8:81063
    Content type: OriginalPaper Published on:

  39. The application of naked DNA containing type I interferon (IFN) transgenes is a promising potential therapeutic approach for controlling chronic viral infections. Herein, we detail the application of this appr...

    Authors: Bobbie Ann Austin, Cassandra M. James, Peter HƤrle and Daniel J. J. Carr
    Citation: Biological Procedures Online 2006 8:81055
    Content type: OriginalPaper Published on:

  40. The Chromatin Immunoprecipiation (ChIP) provides a powerful technique for identifying the in vivo association of transcription factors with regulatory elements. However, obtaining meaningful information for promo...

    Authors: Rebecca L. Dryer and Lori R. Covey
    Citation: Biological Procedures Online 2006 8:81044
    Content type: OriginalPaper Published on:

  41. Microtubule inhibitors such as Vinblastine and Paclitaxel are chemotherapy agents that activate the mitotic spindle checkpoint, arresting cells in mitosis and leading to cell death. The pathways that connect m...

    Authors: Hiroshi Y. Yamada and Gary J. Gorbsky
    Citation: Biological Procedures Online 2006 8:81036
    Content type: OriginalPaper Published on:

  44. Efficient protein folding and quality control are essential for unperturbed cell viability. Defects in these processes may lead to production of aberrant polypeptides that are either degraded leading to ā€œloss-...

    Authors: Tatiana SoldĆ , Silvia Olivari and Maurizio Molinari
    Citation: Biological Procedures Online 2005 7:710136
    Content type: OriginalPaper Published on:

  45. We have developed a simple and effective method (Lig-PCR) for monitoring ligation reactions using PCR and primers that are common to many cloning vectors. Ligation mixtures can directly be used as templates an...

    Authors: Prafulla K. Chandra and Stephen K. Wikel
    Citation: Biological Procedures Online 2005 7:710093
    Content type: OriginalPaper Published on:

  46. Angiogenesis is a complex process involving an ECM and vascular endothelial cells (EC), and is regulated by various angiogenic factors including VEGF. The ability to form a capillary/tube-like network is a spe...

    Authors: Motomu Watanabe, Yayoi Fujioka-Kaneko, Hisayuki Kobayashi, Mamoru Kiniwa, Michihiko Kuwano and Yuji Basaki
    Citation: Biological Procedures Online 2005 7:710041
    Content type: OriginalPaper Published on:

  47. Cell culturing, and the requisite storage of cell lines at ultra-low temperatures, is used in most laboratories studying or using eukaryotic proteomics, genomics, microarray, and RNA technologies. In this stud...

    Authors: Joe Corsini and Ethan Mann
    Citation: Biological Procedures Online 2005 7:710026
    Content type: OriginalPaper Published on:

  48. To assess the role of sortilin in the sorting and trafficking of sphingolipid activator proteins (SAPs) the function of sortilin was abolished by a dominant-negative mutant and by the use of RNAi. Mutant sorti...

    Authors: Stephane Lefrancois, Maryssa Canuel, Jibin Zeng and Carlos R. Morales
    Citation: Biological Procedures Online 2005 7:710017
    Content type: OriginalPaper Published on:

  49. To date, breast cancer (BC) research is mainly studied with cell lines. These cells were passaged multiple times, acquiring phenotypes, additional mutations and epigenetic changes. These changes make the passa...

    Authors: Kelly E. Corcoran, Prem S. Patel and Pranela Rameshwar
    Citation: Biological Procedures Online 2005 7:710008
    Content type: OriginalPaper Published on:
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  • Citation Impact 2023
    Journal Impact Factor: 3.7
    5-year Journal Impact Factor: 4.6
    Source Normalized Impact per Paper (SNIP): 0.960
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Biological Procedures Online

ISSN: 1480-9222

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