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Involvement of integrin-linked kinase in capillary/tube-like network formation of human vascular endothelial cells
Biological Procedures Online volume 7, pages 41–47 (2005)
Angiogenesis is a complex process involving an ECM and vascular endothelial cells (EC), and is regulated by various angiogenic factors including VEGF. The ability to form a capillary/tube-like network is a specialized function of EC. Therefore, in vitro angiogenesis was assessed by a capillary/tube-like network formation assay. There are three angiogenic parameters: capillary length, number of capillaries, and relative capillary area per field. We evaluated capillary length per field in the assay. VEGF promoted capillary/tube-like network formation of EC in a type I collagen gel matrix in vitro. Moreover, we demonstrated the involvement of ILK in a VEGF signaling pathway mediating capillary/tube-like network formation of EC using dominant-negative, kinase deficient ILK. This is a straightforward assay to monitor responses of human vascular endothelial cells.
human umbilical vein endothelial cells
- PI 3-kinase:
protein kinase B
vascular endothelial growth factor
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Watanabe and Basaki contributed equally to this manuscript.
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Watanabe, M., Fujioka-Kaneko, Y., Kobayashi, H. et al. Involvement of integrin-linked kinase in capillary/tube-like network formation of human vascular endothelial cells. Biol. Proced. Online 7, 41–47 (2005). https://doi.org/10.1251/bpo104
- Endothelial Cells
- Vascular Endothelial Growth Factor A
- Integrin-Linked Kinase