Open Access

Involvement of integrin-linked kinase in capillary/tube-like network formation of human vascular endothelial cells

  • Motomu Watanabe1,
  • Yayoi Fujioka-Kaneko2,
  • Hisayuki Kobayashi3,
  • Mamoru Kiniwa1,
  • Michihiko Kuwano4, 5 and
  • Yuji Basaki6, 7, 8Email author
Biological Procedures Online7:710041

https://doi.org/10.1251/bpo104

Received: 16 December 2004

Accepted: 28 March 2005

Abstract

Angiogenesis is a complex process involving an ECM and vascular endothelial cells (EC), and is regulated by various angiogenic factors including VEGF. The ability to form a capillary/tube-like network is a specialized function of EC. Therefore, in vitro angiogenesis was assessed by a capillary/tube-like network formation assay. There are three angiogenic parameters: capillary length, number of capillaries, and relative capillary area per field. We evaluated capillary length per field in the assay. VEGF promoted capillary/tube-like network formation of EC in a type I collagen gel matrix in vitro. Moreover, we demonstrated the involvement of ILK in a VEGF signaling pathway mediating capillary/tube-like network formation of EC using dominant-negative, kinase deficient ILK. This is a straightforward assay to monitor responses of human vascular endothelial cells.

Indexing terms

Endothelial Cells Vascular Endothelial Growth Factor A Integrin-Linked Kinase

Notes

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