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Involvement of integrin-linked kinase in capillary/tube-like network formation of human vascular endothelial cells

Abstract

Angiogenesis is a complex process involving an ECM and vascular endothelial cells (EC), and is regulated by various angiogenic factors including VEGF. The ability to form a capillary/tube-like network is a specialized function of EC. Therefore, in vitro angiogenesis was assessed by a capillary/tube-like network formation assay. There are three angiogenic parameters: capillary length, number of capillaries, and relative capillary area per field. We evaluated capillary length per field in the assay. VEGF promoted capillary/tube-like network formation of EC in a type I collagen gel matrix in vitro. Moreover, we demonstrated the involvement of ILK in a VEGF signaling pathway mediating capillary/tube-like network formation of EC using dominant-negative, kinase deficient ILK. This is a straightforward assay to monitor responses of human vascular endothelial cells.

Abbreviations

ECM:

extracellular matrix

HUVEC:

human umbilical vein endothelial cells

ILK:

integrin-linked kinase

KD:

kinase deficient

PI 3-kinase:

phosphatidylinositol 3-kinase

PKB:

protein kinase B

VEGF:

vascular endothelial growth factor

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Correspondence to Yuji Basaki Ph.D..

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Watanabe and Basaki contributed equally to this manuscript.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Watanabe, M., Fujioka-Kaneko, Y., Kobayashi, H. et al. Involvement of integrin-linked kinase in capillary/tube-like network formation of human vascular endothelial cells. Biol. Proced. Online 7, 41–47 (2005). https://doi.org/10.1251/bpo104

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  • DOI: https://doi.org/10.1251/bpo104

Indexing terms

  • Endothelial Cells
  • Vascular Endothelial Growth Factor A
  • Integrin-Linked Kinase