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  1. Content type: Research

    Tibial dyschondroplasia (TD) is a skeletal disease of fast growing chicken and other avian species. It is characterized by an avascular and non-mineralized growth plate, which leads to a deformed tibial bone a...

    Authors: Mujahid Iqbal, Hui Zhang, Khalid Mehmood, Aoyun Li, Xiong Jiang, Yaping Wang, Jialu Zhang, Muhammad Kashif Iqbal, Mujeeb Ur Rehman, Wangyuan Yao, Shijin Yang and Jiakui Li

    Citation: Biological Procedures Online 2018 20:15

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  2. Content type: Methodology

    Although genomic DNA isolation using the Chelex 100 resin is rapid and inexpensive, the DNA obtained by this method has a low concentration in solution and contains suspended impurities. The presence of debris...

    Authors: Utkarsha A. Singh, Mukta Kumari and Soumya Iyengar

    Citation: Biological Procedures Online 2018 20:12

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  3. Content type: Research

    Kochia scoparia is a dicotyledonous annual herb and belongs to the Amaranthaceae family. Genetic diversity and resistance to drought stress of this plant has made it widely scattered in different regions which co...

    Authors: Leila Fahmideh and Ziba Fooladvand

    Citation: Biological Procedures Online 2018 20:11

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  4. Content type: Research

    Gastric cancer is currently the fourth leading cause of cancer-related death worldwide. Gastric cancer is often diagnosed at advanced stages and the outcome of the treatment is often poor. Therefore, identifyi...

    Authors: Yu-Chang Chuang, Hsin-Yi Wu, Yu-Ling Lin, Shey-Cherng Tzou, Cheng-Hsun Chuang, Ting-Yan Jian, Pin-Rong Chen, Yuan-Ching Chang, Chi-Hsin Lin, Tse-Hung Huang, Chao-Ching Wang, Yi-Lin Chan and Kuang-Wen Liao

    Citation: Biological Procedures Online 2018 20:10

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  5. Content type: Methodology

    Soil bacterium Sinorhizobium meliloti (S. meliloti) forms an endosymbiotic partnership with Medicago truncatula (M. truncatula) roots which results in root nodules. The bacteria live within root nodules where the...

    Authors: Mengsheng Gao, Anne Benge, Julia M. Mesa, Regina Javier and Feng-Xia Liu

    Citation: Biological Procedures Online 2018 20:8

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  6. Content type: Research

    Neutrophil extracellular traps (NETs), extracellular structures composed of decondensed chromatin and antimicrobial molecules, are released in a process called NETosis. NETs, which are part of normal host defe...

    Authors: Ryan Rebernick, Lauren Fahmy, Christopher Glover, Mandar Bawadekar, Daeun Shim, Caitlyn L. Holmes, Nicole Rademacher, Hemanth Potluri, Christie M. Bartels and Miriam A. Shelef

    Citation: Biological Procedures Online 2018 20:7

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  7. Content type: Research

    To evaluate the effect of qi-nourishing essence-replenishing Chinese herbal medicine combined with chemotherapy in survival of advanced non-small-cell lung cancer(NSCLC) patients with essence and qi deficiency.

    Authors: Yabin Gong, Zhenye Xu, Changjuan Jin, Haibin Deng, Zhongqi Wang, Weidong Zhou, Ming Zhang, Xiaozhen Zhao and Lifang Wang

    Citation: Biological Procedures Online 2018 20:9

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  8. Content type: Methodology

    Subcellular localization of coding and non-coding RNAs has emerged as major regulatory mechanisms of gene expression in various cell types and many organisms. However, techniques that enable detection of the s...

    Authors: Natsumi Takei, Takuma Nakamura, Shohei Kawamura, Yuki Takada, Yui Satoh, Atsushi P. Kimura and Tomoya Kotani

    Citation: Biological Procedures Online 2018 20:6

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  9. Content type: Research

    Hierarchical Sample clustering (HSC) is widely performed to examine associations within expression data obtained from microarrays and RNA sequencing (RNA-seq). Researchers have investigated the HSC results wit...

    Authors: Shitao Zhao, Jianqiang Sun, Kentaro Shimizu and Koji Kadota

    Citation: Biological Procedures Online 2018 20:5

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  10. Content type: Research

    Dendritic cells (DCs), which can be used as anti-cancer vaccines, are generally obtained in vitro from isolated CD14+ monocytes (MoDCs). This generates high cell numbers and allows instructing DCs to guarantee ef...

    Authors: Graça S Marques, Zélia Silva and Paula A. Videira

    Citation: Biological Procedures Online 2018 20:4

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  11. Content type: Methodology

    Apically located tight junctions in airway epithelium perform a fundamental role in controlling macromolecule migration through paracellular spaces. Alterations in their expression may lead to disruptions in b...

    Authors: Alysia G. Buckley, Kevin Looi, Thomas Iosifidis, Kak-Ming Ling, Erika N. Sutanto, Kelly M. Martinovich, Elizabeth Kicic-Starcevich, Luke W. Garratt, Nicole C. Shaw, Francis J. Lannigan, Alexander N. Larcombe, Graeme Zosky, Darryl A. Knight, Paul J. Rigby, Anthony Kicic and Stephen M. Stick

    Citation: Biological Procedures Online 2018 20:3

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  12. Content type: Review

    Traditional Chinese medicine (TCM) plays an indispensable role in cancer prevention and treatment. Chinese herbal medicine (CHM) is a key component of TCM and has been practiced for thousands of years. A numbe...

    Authors: Lijing Jiao, Ling Bi, Yan Lu, Qin Wang, Yabin Gong, Jun Shi and Ling Xu

    Citation: Biological Procedures Online 2018 20:1

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  13. Content type: Methodology

    Transcriptomic profiling of ex vivo cultured human limbal epithelial stem cells (hLESCs) will foster better understanding of corneal physiology and novel treatment paradigms to limbal stem cell deficiency (LSC...

    Authors: Lei Liu, Frederik Mølgaard Nielsen, Simone Elkjær Riis, Jeppe Emmersen, Trine Fink, Jesper Østergaard Hjortdal, Chris Bath and Vladimir Zachar

    Citation: Biological Procedures Online 2017 19:15

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  14. Content type: Methodology

    Human trefoil factor (TFF) peptides consist of three members: TFF1, TFF2 and TFF3. TFF3 is the most abundant TFF peptide in saliva. TFF3 homodimer was suggested to be involved in apoptosis inhibition and malig...

    Authors: Saichit Khummuang, Waraporn Phanphrom, Witida Laopajon, Watchara Kasinrerk, Ponlatham Chaiyarit and Supansa Pata

    Citation: Biological Procedures Online 2017 19:14

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  15. Content type: Research

    Somatic cell nuclear transfer (SCNT) is a useful biotechnological tool for transgenic animal production using genetically modified somatic cells (GMSCs). However, there are several limitations preventing succe...

    Authors: Kiyoung Eun, Seon-Ung Hwang, Yeon Woo Jeong, Sunyoung Seo, Seon Yong Lee, Woo Suk Hwang, Sang-Hwan Hyun and Hyunggee Kim

    Citation: Biological Procedures Online 2017 19:13

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  16. Content type: Short report

    Site-directed mutagenesis (SDM) has been widely used for studying the structure and function of proteins. A one-step polymerase chain reaction (PCR)-based multiple site-directed plasmid mutagenesis method with...

    Authors: Ting-Yu Kuo, Chung-Che Tsai and Hua-Wen Fu

    Citation: Biological Procedures Online 2017 19:12

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  17. Content type: Methodology

    Immunotherapy is a very fast expanding field within drug discovery and, hence, rapid and inexpensive expression of antibodies would be extremely valuable. Antibodies are, however, difficult to express. Multifu...

    Authors: Xiaotian T. Fang, Dag Sehlin, Lars Lannfelt, Stina Syvänen and Greta Hultqvist

    Citation: Biological Procedures Online 2017 19:11

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  18. Content type: Research

    Genomic loci associated with histone marks are typically analyzed by immunoprecipitation of the chromatin followed by quantitative-PCR (ChIP-qPCR) or high throughput sequencing (ChIP-seq). Chromatin can be eit...

    Authors: Sarah-Anne David, Benoît Piégu, Christelle Hennequet-Antier, Maëlle Pannetier, Tiphaine Aguirre-Lavin, Sabine Crochet, Thierry Bordeau, Nathalie Couroussé, Aurélien Brionne, Yves Bigot, Anne Collin and Vincent Coustham

    Citation: Biological Procedures Online 2017 19:10

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  19. Content type: Methodology

    Primordial germ cells (PGC) are the precursors of the gametes. During pre-natal development, PGC undergo an epigenetic reprogramming when bulk DNA demethylation occurs and is followed by sex-specific de novo m...

    Authors: Isabelle Hernandez Cantão, Renato Borges Tesser and Taiza Stumpp

    Citation: Biological Procedures Online 2017 19:9

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  20. Content type: Methodology

    Whatever the target of an experiment in cell biology, cell counting and viability assessment are always computed. The Trypan Blue (TB) assay was proposed about a century ago and is still the most widely used m...

    Authors: Filippo Piccinini, Anna Tesei, Chiara Arienti and Alessandro Bevilacqua

    Citation: Biological Procedures Online 2017 19:8

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  21. Content type: Methodology

    Pathological analyses and methodology has recently undergone a dramatic revolution. With the creation of tissue clearing methods such as CLARITY and CUBIC, groups can now achieve complete transparency in tissu...

    Authors: Sean J. Miller and Jeffrey D. Rothstein

    Citation: Biological Procedures Online 2017 19:7

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  22. Content type: Research

    In vitro labelling of cells and small cell structures is a necessary step before in vivo monitoring of grafts. We modified and optimised a procedure for pancreatic islet labelling using bimodal positively char...

    Authors: Vít Herynek, Andrea Gálisová, Mangala Srinivas, Eric A. W. van Dinther, Lucie Kosinová, Jiri Ruzicka, Markéta Jirátová, Jan Kriz and Daniel Jirák

    Citation: Biological Procedures Online 2017 19:6

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  23. Content type: Research

    Reduced representation bisulfite sequencing (RRBS) has been widely used to profile genome-scale DNA methylation in mammalian genomes. However, the applications and technical performances of RRBS with different...

    Authors: Xiao-Long Yuan, Zhe Zhang, Rong-Yang Pan, Ning Gao, Xi Deng, Bin Li, Hao Zhang, Per Torp Sangild and Jia-Qi Li

    Citation: Biological Procedures Online 2017 19:5

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