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Methodology
Primordial germ cells (PGC) are the precursors of the gametes. During pre-natal development, PGC undergo an epigenetic reprogramming when bulk DNA demethylation occurs and is followed by sex-specific de novo m...
Biological Procedures Online 2017 19:9
Published on: 3 August 2017
Methodology
Whatever the target of an experiment in cell biology, cell counting and viability assessment are always computed. The Trypan Blue (TB) assay was proposed about a century ago and is still the most widely used m...
Biological Procedures Online 2017 19:8
Published on: 20 July 2017
Methodology
Pathological analyses and methodology has recently undergone a dramatic revolution. With the creation of tissue clearing methods such as CLARITY and CUBIC, groups can now achieve complete transparency in tissu...
Biological Procedures Online 2017 19:7
Published on: 5 July 2017
Research
In vitro labelling of cells and small cell structures is a necessary step before in vivo monitoring of grafts. We modified and optimised a procedure for pancreatic islet labelling using bimodal positively char...
Biological Procedures Online 2017 19:6
Published on: 28 June 2017
Research
Reduced representation bisulfite sequencing (RRBS) has been widely used to profile genome-scale DNA methylation in mammalian genomes. However, the applications and technical performances of RRBS with different...
Biological Procedures Online 2017 19:5
Published on: 7 June 2017
Methodology
TER measurements across confluent cellular monolayers provide a useful indication of TJ strength between epithelial and endothelial cells in culture. Having a reliable and accurate method of measuring cell-to-...
Biological Procedures Online 2017 19:4
Published on: 10 May 2017
Review
Immune responses combat various infectious agents by inducing inflammatory responses, antimicrobial pathways and adaptive immunity. The polygenic responses to these external stimuli are temporally and coordina...
Biological Procedures Online 2017 19:3
Published on: 27 April 2017
Review
DNA/RNA methylation plays an important role in lung cancer initiation and progression. Liquid biopsy makes use of cells, nucleotides and proteins released from tumor cells into body fluids to help with cancer ...
Biological Procedures Online 2017 19:2
Published on: 17 March 2017
Methodology
Indole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used...
Biological Procedures Online 2017 19:1
Published on: 13 January 2017
Methodology
Cells are continuously exposed to changes in their environment. Endocrine systems, in particular, communicate by rhythms and feedback loops. In this study, we developed an automated system to produce such cond...
Biological Procedures Online 2016 18:19
Published on: 21 November 2016
Review
Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membran...
Biological Procedures Online 2016 18:18
Published on: 28 October 2016
Methodology
The immunoprecipitation (IP) assay is a valuable molecular biology tool applied across a breadth of fields. The standard assay couples IP to immunoblotting (IP/IB), a procedure severely limited as it is not ea...
Biological Procedures Online 2016 18:16
Published on: 15 August 2016
Research
A key requirements for therapy utilizing the tissue engineering methodologies is use of techniques which have the capability to yield a high number of cells, from small tissue biopsy in a relatively short time...
Biological Procedures Online 2016 18:17
Published on: 12 August 2016
Methodology
The skin is the largest organ of the human body and is the first line barrier defence against trauma, microbial infiltration and radiation. Skin diseases can be a result of multi-systemic disease or an isolate...
Biological Procedures Online 2016 18:15
Published on: 21 July 2016
Methodology
A number of integrase defective lentiviral (IDLV) packaging systems have been developed to produce integration deficient lentiviruses for gene delivery and epichromosomal expression. However, despite their gro...
Biological Procedures Online 2016 18:14
Published on: 11 July 2016
Methodology
Satisfactory sample preparation for mass spectrometry-based analysis is a critical step in the proteomics workflow. The quality and reproducibility of sample preparation can determine the coverage and confiden...
Biological Procedures Online 2016 18:13
Published on: 21 June 2016
Expression of Concern
Biological Procedures Online 2016 18:12
Published on: 9 June 2016
The original article was published in Biological Procedures Online 2014 16:42
Review
The number of species with completed genomes, including those with evidence for recent whole genome duplication events has exploded. The recently sequenced Atlantic salmon genome has been through two rounds of...
Biological Procedures Online 2016 18:11
Published on: 10 May 2016
Short report
Endothelial-Mesenchymal-Transition (EndMT) plays an essential role in cardiovascular development, and recently became an attractive therapeutic target based on evidence supporting its involvement in fibrosis a...
Biological Procedures Online 2016 18:10
Published on: 27 April 2016
Research
2’-5’ oligoadenylate synthetases (OAS) are interferon inducible enzymes that polymerizes ATP to 2’-5’-linked oligomers of adenylate (2-5As). As part of the innate immune response, these enzymes are activated b...
Biological Procedures Online 2016 18:9
Published on: 17 March 2016
Review
Stem cells are unspecialized/undifferentiated cells that exist in embryos and adult tissues or can be converted from somatic differentiated cells. Use of stem cells for tissue regeneration and tissue engineeri...
Biological Procedures Online 2016 18:8
Published on: 9 March 2016
Reviewer acknowledgement
A peer-reviewed journal would not survive without the generous time and insightful comments of the reviewers, whose efforts often go unrecognized. Although final decisions are always editorial, they are greatl...
Biological Procedures Online 2016 18:7
Published on: 3 March 2016
Methodology
In vitro investigations of membrane proteins usually depend on detergents for protein solubilisation and stabilisation. The amount of detergent bound to a membrane protein is relevant to successful experiment ...
Biological Procedures Online 2016 18:4
Published on: 15 February 2016
Short report
Circular plasmid-mediated homologous recombination is commonly used for marker-less allelic replacement, exploiting the endogenous recombination machinery of the host. Common limitations of existing methods in...
Biological Procedures Online 2016 18:6
Published on: 13 February 2016
Methodology
The ability to introduce site-specific mutations in bacterial pathogens is essential towards understanding their molecular mechanisms of pathogenicity. This has been greatly facilitated by the genetic engineer...
Biological Procedures Online 2016 18:3
Published on: 3 February 2016
