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Cell-based expression cloning for identification of polypeptides that hypersensitize mammalian cells to mitotic arrest
Biological Procedures Online volume 8, pages 36–43 (2006)
Abstract
Microtubule inhibitors such as Vinblastine and Paclitaxel are chemotherapy agents that activate the mitotic spindle checkpoint, arresting cells in mitosis and leading to cell death. The pathways that connect mitotic arrest to cell death are not well characterized. We developed a mammalian cell-based cDNA cloning method to isolate proteins and protein fragments whose expression inhibits colony formation in the presence of microtubule inhibitors. Understanding how these proteins impact cellular responses to microtubule drugs will lead to better understanding of the biochemical pathways connecting mitotic arrest and cell death in mammalian cells and may provide novel targets that can enhance microtubule inhibitor-mediated chemotherapy.
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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Yamada, H.Y., Gorbsky, G.J. Cell-based expression cloning for identification of polypeptides that hypersensitize mammalian cells to mitotic arrest. Biol. Proced. Online 8, 36–43 (2006). https://doi.org/10.1251/bpo116
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DOI: https://doi.org/10.1251/bpo116