Open Access

Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array

  • Seok Hwee Koo1,
  • Tan Ching Ong2,
  • Kok Ting Chong1,
  • Caroline Guat Lay Lee3,
  • Fook Tim Chew2 and
  • Edmund Jon Deoon Lee1Email author
Biological Procedures Online9:91027

https://doi.org/10.1251/bpo131

Received: 12 March 2007

Accepted: 3 July 2007

Abstract

We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cassette transporters. Genetic polymorphisms have been known to influence therapeutic response and risk of disease pathologies. Genetic screening for therapeutic and diagnostic applications thus holds great promise in clinical management. The allele-specific primer extension (ASPE) reaction was used to assay 22 multiplexed SNPs for eight subjects. Comparison of the microsphere-based ASPE assay results to sequencing results showed complete concordance in genotype assignments. The Bioplex suspension array thus proves to be a reliable, cost-effective and high-throughput technological platform for genotyping. It can be easily adapted to customized SNP panels for specific applications involving large-scale mutation screening of clinically relevant markers.

Indexing terms

GenotypeMicrospheresPolymorphism, Genetic

Advertisement