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Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array


We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cassette transporters. Genetic polymorphisms have been known to influence therapeutic response and risk of disease pathologies. Genetic screening for therapeutic and diagnostic applications thus holds great promise in clinical management. The allele-specific primer extension (ASPE) reaction was used to assay 22 multiplexed SNPs for eight subjects. Comparison of the microsphere-based ASPE assay results to sequencing results showed complete concordance in genotype assignments. The Bioplex suspension array thus proves to be a reliable, cost-effective and high-throughput technological platform for genotyping. It can be easily adapted to customized SNP panels for specific applications involving large-scale mutation screening of clinically relevant markers.



ATP-binding cassette


allele-specific primer extension


1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride


2[N-Morpholino] ethanesulfonic acid


median fluorescence intensity


shrimp alkaline phosphatase


sodium dodecyl sulfate


single nucleotide polymorphism


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Correspondence to Edmund Jon Deoon Lee.

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Koo, S.H., Ong, T.C., Chong, K.T. et al. Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array. Biol. Proced. Online 9, 27–42 (2007).

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Indexing terms

  • Genotype
  • Microspheres
  • Polymorphism, Genetic