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Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array
Biological Procedures Online volume 9, pages 27–42 (2007)
We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cassette transporters. Genetic polymorphisms have been known to influence therapeutic response and risk of disease pathologies. Genetic screening for therapeutic and diagnostic applications thus holds great promise in clinical management. The allele-specific primer extension (ASPE) reaction was used to assay 22 multiplexed SNPs for eight subjects. Comparison of the microsphere-based ASPE assay results to sequencing results showed complete concordance in genotype assignments. The Bioplex suspension array thus proves to be a reliable, cost-effective and high-throughput technological platform for genotyping. It can be easily adapted to customized SNP panels for specific applications involving large-scale mutation screening of clinically relevant markers.
allele-specific primer extension
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride
2[N-Morpholino] ethanesulfonic acid
median fluorescence intensity
shrimp alkaline phosphatase
sodium dodecyl sulfate
single nucleotide polymorphism
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Koo, S.H., Ong, T.C., Chong, K.T. et al. Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array. Biol. Proced. Online 9, 27–42 (2007). https://doi.org/10.1251/bpo131
- Polymorphism, Genetic