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Quantitative evaluation of signaling events in Drosophila S2 cells

Biological Procedures Online volume 10, pages 20–28 (2008)Cite this article

Abstract

Drosophila activates a robust defense response to gram-negative bacteria through the Immune deficiency (Imd) pathway. Imd signaling proceeds through c-Jun N-terminal Kinase (JNK), NF-kB and caspase modules. The individual signaling modules act in a highly coordinated manner to yield a stereotypical response to infection. While considerable attention has focused on NF-kB-mediated antimicrobial activities, more recent studies have highlighted the involvement of JNK signaling in the Imd pathway response. JNK signaling occurs in a transitory burst and drives the expression of a number of gene products through the AP-1 transcription factor. In this report, we describe a simple method for the quantification of JNK activation by Western blot analysis or directly in tissue culture plates.

Abbreviations

IKK:

I-kappa kinase

Imd:

immune deficiency

JNK:

c-Jun N-terminal kinase

PGN:

gram-negative bacterial peptidoglycan

PGRP-LC:

peptidoglycan recognition protein-LC

TNF:

tumor necrosis factor

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Authors and Affiliations

  1. Department of Medical Microbiology and Immunology, University of Alberta, T6G 2S2, Edmonton, AB, Canada

    David Bond, David A. Primrose & Edan Foley

Authors
  1. David Bond
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  2. David A. Primrose
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  3. Edan Foley
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Correspondence to Edan Foley.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bond, D., Primrose, D.A. & Foley, E. Quantitative evaluation of signaling events in Drosophila S2 cells. Biol. Proced. Online 10, 20–28 (2008). https://doi.org/10.1251/bpo139

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  • DOI: https://doi.org/10.1251/bpo139

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Biological Procedures Online

ISSN: 1480-9222

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