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Chromatin immunoprecipitation assay detects er recruitment to gene specific promoters in uterus

Abstract

Chromatin immunoprecipitation (ChIP) technique allows detection of proteins that bind to chromatin. While this technique has been applied extensively in cell-based studies, its tissue-based application remains poorly explored. We are specifically interested in examining estrogen-dependent transcriptional mechanism in respect of recruitment of estrogen receptor-alpha (ERα), a ligand-activated transcription factor, to uterine gene promoters in mice. Recent genearray studies, utilizing ERα knock-out vs. wild-type mice, have revealed that estrogen regulates numerous uterine genes temporally and most importantly via ERα during the phase-II response, including three well characterized genes viz., lactoferrin (Ltf), progesterone receptor (Pgr) and cyclinD1 (Ccnd1). Here, utilizing systematic ChIP studies, we demonstrate endogenous recruitment of ERα to above uterine gene promoters following estradiol-17β (E2) injection in mice.

Abbreviations

ChIP:

chromatin immunoprecipitation

ERα:

estrogen receptor-alpha

Ltf :

lactoferrin

Pgr :

progesterone receptor

Ccnd1:

cyclin D1

IP:

immunoprecipitation

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Correspondence to Sanjoy K. Das.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ray, S., Das, S.K. Chromatin immunoprecipitation assay detects er recruitment to gene specific promoters in uterus. Biol. Proced. Online 8, 69–76 (2006). https://doi.org/10.1251/bpo120

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  • DOI: https://doi.org/10.1251/bpo120

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