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  1. Research

    SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo

    Somatic cell nuclear transfer (SCNT) is a useful biotechnological tool for transgenic animal production using genetically modified somatic cells (GMSCs). However, there are several limitations preventing succe...

    Kiyoung Eun, Seon-Ung Hwang, Yeon Woo Jeong, Sunyoung Seo, Seon Yong Lee, Woo Suk Hwang, Sang-Hwan Hyun and Hyunggee Kim

    Biological Procedures Online 2017 19:13

    Published on: 18 October 2017

  2. Short report

    Enhanced Mutant Screening in One-step PCR-based Multiple Site-directed Plasmid Mutagenesis by Introduction of Silent Restriction Sites for Structural and Functional Study of Proteins

    Site-directed mutagenesis (SDM) has been widely used for studying the structure and function of proteins. A one-step polymerase chain reaction (PCR)-based multiple site-directed plasmid mutagenesis method with...

    Ting-Yu Kuo, Chung-Che Tsai and Hua-Wen Fu

    Biological Procedures Online 2017 19:12

    Published on: 26 September 2017

  3. Research

    An Assessment of Fixed and Native Chromatin Preparation Methods to Study Histone Post-Translational Modifications at a Whole Genome Scale in Skeletal Muscle Tissue

    Genomic loci associated with histone marks are typically analyzed by immunoprecipitation of the chromatin followed by quantitative-PCR (ChIP-qPCR) or high throughput sequencing (ChIP-seq). Chromatin can be eit...

    Sarah-Anne David, Benoît Piégu, Christelle Hennequet-Antier, Maëlle Pannetier, Tiphaine Aguirre-Lavin, Sabine Crochet, Thierry Bordeau, Nathalie Couroussé, Aurélien Brionne, Yves Bigot, Anne Collin and Vincent Coustham

    Biological Procedures Online 2017 19:10

    Published on: 29 August 2017

  4. Research

    Pre-Microporation Improves Outcome of Pancreatic Islet Labelling for Optical and 19F MR Imaging

    In vitro labelling of cells and small cell structures is a necessary step before in vivo monitoring of grafts. We modified and optimised a procedure for pancreatic islet labelling using bimodal positively char...

    Vít Herynek, Andrea Gálisová, Mangala Srinivas, Eric A. W. van Dinther, Lucie Kosinová, Jiri Ruzicka, Markéta Jirátová, Jan Kriz and Daniel Jirák

    Biological Procedures Online 2017 19:6

    Published on: 28 June 2017

  5. Research

    Performances of Different Fragment Sizes for Reduced Representation Bisulfite Sequencing in Pigs

    Reduced representation bisulfite sequencing (RRBS) has been widely used to profile genome-scale DNA methylation in mammalian genomes. However, the applications and technical performances of RRBS with different...

    Xiao-Long Yuan, Zhe Zhang, Rong-Yang Pan, Ning Gao, Xi Deng, Bin Li, Hao Zhang, Per Torp Sangild and Jia-Qi Li

    Biological Procedures Online 2017 19:5

    Published on: 7 June 2017

  6. Review

    LncRNAs and immunity: watchdogs for host pathogen interactions

    Immune responses combat various infectious agents by inducing inflammatory responses, antimicrobial pathways and adaptive immunity. The polygenic responses to these external stimuli are temporally and coordina...

    Peerzada Tajamul Mumtaz, Shakil Ahmad Bhat, Syed Mudasir Ahmad, Mashooq Ahmad Dar, Raashid Ahmed, Uneeb Urwat, Aadil Ayaz, Divya Shrivastava, Riaz Ahmad Shah and Nazir Ahmad Ganai

    Biological Procedures Online 2017 19:3

    Published on: 27 April 2017

  7. Review

    Methylated DNA/RNA in Body Fluids as Biomarkers for Lung Cancer

    DNA/RNA methylation plays an important role in lung cancer initiation and progression. Liquid biopsy makes use of cells, nucleotides and proteins released from tumor cells into body fluids to help with cancer ...

    Yan Lu, Shulin/SL Li, Shiguo/SG Zhu, Yabin/YB Gong, Jun/J Shi and Ling/ L Xu

    Biological Procedures Online 2017 19:2

    Published on: 17 March 2017

  8. Methodology

    Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins

    Indole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used...

    Yi Su, Weigui Luo, Xiaofei Chen, Huizhen Liu, Yueqing Hu, Wanhuang Lin and Langtao Xiao

    Biological Procedures Online 2017 19:1

    Published on: 13 January 2017

  9. Methodology

    An Automated Perifusion System for Modifying Cell Culture Conditions over Time

    Cells are continuously exposed to changes in their environment. Endocrine systems, in particular, communicate by rhythms and feedback loops. In this study, we developed an automated system to produce such cond...

    Nicholas B. Whitticar, Elisha W. Strahler, Parthiban Rajan, Savas Kaya and Craig S. Nunemaker

    Biological Procedures Online 2016 18:19

    Published on: 21 November 2016

  10. Review

    Aqueous two-phase system (ATPS): an overview and advances in its applications

    Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membran...

    Mujahid Iqbal, Yanfei Tao, Shuyu Xie, Yufei Zhu, Dongmei Chen, Xu Wang, Lingli Huang, Dapeng Peng, Adeel Sattar, Muhammad Abu Bakr Shabbir, Hafiz Iftikhar Hussain, Saeed Ahmed and Zonghui Yuan

    Biological Procedures Online 2016 18:18

    Published on: 28 October 2016

  11. Methodology

    Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP

    The immunoprecipitation (IP) assay is a valuable molecular biology tool applied across a breadth of fields. The standard assay couples IP to immunoblotting (IP/IB), a procedure severely limited as it is not ea...

    Paolo Mita, Tenzin Lhakhang, Donghui Li, Daniel J. Eichinger, David Fenyo and Jef D. Boeke

    Biological Procedures Online 2016 18:16

    Published on: 15 August 2016

  12. Research

    Isolation, expansion and characterization of porcine urinary bladder smooth muscle cells for tissue engineering

    A key requirements for therapy utilizing the tissue engineering methodologies is use of techniques which have the capability to yield a high number of cells, from small tissue biopsy in a relatively short time...

    Marta Pokrywczynska, Daria Balcerczyk, Arkadiusz Jundzill, Maciej Gagat, Monika Czapiewska, Tomasz Kloskowski, Maciej Nowacki, Agata M. Gastecka, Magdalena Bodnar, Alina Grzanka, Andrzej Marszalek and Tomasz Drewa

    Biological Procedures Online 2016 18:17

    Published on: 12 August 2016

  13. Methodology

    An optimised method for the proteomic profiling of full thickness human skin

    The skin is the largest organ of the human body and is the first line barrier defence against trauma, microbial infiltration and radiation. Skin diseases can be a result of multi-systemic disease or an isolate...

    Emily Bliss, Wendy E. Heywood, Malika Benatti, Neil J. Sebire and Kevin Mills

    Biological Procedures Online 2016 18:15

    Published on: 21 July 2016

  14. Methodology

    An Optimized Protocol for Packaging Pseudotyped Integrase Defective Lentivirus

    A number of integrase defective lentiviral (IDLV) packaging systems have been developed to produce integration deficient lentiviruses for gene delivery and epichromosomal expression. However, despite their gro...

    Ranjita Sengupta, Chandreyee Mukherjee, Nandita Sarkar, Zhihong Sun, Jacob Lesnik, Joseph Huang and Biao Lu

    Biological Procedures Online 2016 18:14

    Published on: 11 July 2016

  15. Methodology

    Reliable FASP-based procedures for optimal quantitative proteomic and phosphoproteomic analysis on samples from acute myeloid leukemia patients

    Satisfactory sample preparation for mass spectrometry-based analysis is a critical step in the proteomics workflow. The quality and reproducibility of sample preparation can determine the coverage and confiden...

    Maria Hernandez-Valladares, Elise Aasebø, Olav Mjaavatten, Marc Vaudel, Øystein Bruserud, Frode Berven and Frode Selheim

    Biological Procedures Online 2016 18:13

    Published on: 21 June 2016

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