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  1. Research

    Pre-Microporation Improves Outcome of Pancreatic Islet Labelling for Optical and 19F MR Imaging

    In vitro labelling of cells and small cell structures is a necessary step before in vivo monitoring of grafts. We modified and optimised a procedure for pancreatic islet labelling using bimodal positively char...

    Vít Herynek, Andrea Gálisová, Mangala Srinivas, Eric A. W. van Dinther, Lucie Kosinová, Jiri Ruzicka, Markéta Jirátová, Jan Kriz and Daniel Jirák

    Biological Procedures Online 2017 19:6

    Published on: 28 June 2017

  2. Research

    Performances of Different Fragment Sizes for Reduced Representation Bisulfite Sequencing in Pigs

    Reduced representation bisulfite sequencing (RRBS) has been widely used to profile genome-scale DNA methylation in mammalian genomes. However, the applications and technical performances of RRBS with different...

    Xiao-Long Yuan, Zhe Zhang, Rong-Yang Pan, Ning Gao, Xi Deng, Bin Li, Hao Zhang, Per Torp Sangild and Jia-Qi Li

    Biological Procedures Online 2017 19:5

    Published on: 7 June 2017

  3. Review

    LncRNAs and immunity: watchdogs for host pathogen interactions

    Immune responses combat various infectious agents by inducing inflammatory responses, antimicrobial pathways and adaptive immunity. The polygenic responses to these external stimuli are temporally and coordina...

    Peerzada Tajamul Mumtaz, Shakil Ahmad Bhat, Syed Mudasir Ahmad, Mashooq Ahmad Dar, Raashid Ahmed, Uneeb Urwat, Aadil Ayaz, Divya Shrivastava, Riaz Ahmad Shah and Nazir Ahmad Ganai

    Biological Procedures Online 2017 19:3

    Published on: 27 April 2017

  4. Review

    Methylated DNA/RNA in Body Fluids as Biomarkers for Lung Cancer

    DNA/RNA methylation plays an important role in lung cancer initiation and progression. Liquid biopsy makes use of cells, nucleotides and proteins released from tumor cells into body fluids to help with cancer ...

    Yan Lu, Shulin/SL Li, Shiguo/SG Zhu, Yabin/YB Gong, Jun/J Shi and Ling/ L Xu

    Biological Procedures Online 2017 19:2

    Published on: 17 March 2017

  5. Methodology

    Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins

    Indole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used...

    Yi Su, Weigui Luo, Xiaofei Chen, Huizhen Liu, Yueqing Hu, Wanhuang Lin and Langtao Xiao

    Biological Procedures Online 2017 19:1

    Published on: 13 January 2017

  6. Methodology

    An Automated Perifusion System for Modifying Cell Culture Conditions over Time

    Cells are continuously exposed to changes in their environment. Endocrine systems, in particular, communicate by rhythms and feedback loops. In this study, we developed an automated system to produce such cond...

    Nicholas B. Whitticar, Elisha W. Strahler, Parthiban Rajan, Savas Kaya and Craig S. Nunemaker

    Biological Procedures Online 2016 18:19

    Published on: 21 November 2016

  7. Review

    Aqueous two-phase system (ATPS): an overview and advances in its applications

    Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membran...

    Mujahid Iqbal, Yanfei Tao, Shuyu Xie, Yufei Zhu, Dongmei Chen, Xu Wang, Lingli Huang, Dapeng Peng, Adeel Sattar, Muhammad Abu Bakr Shabbir, Hafiz Iftikhar Hussain, Saeed Ahmed and Zonghui Yuan

    Biological Procedures Online 2016 18:18

    Published on: 28 October 2016

  8. Methodology

    Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for High-Throughput IP

    The immunoprecipitation (IP) assay is a valuable molecular biology tool applied across a breadth of fields. The standard assay couples IP to immunoblotting (IP/IB), a procedure severely limited as it is not ea...

    Paolo Mita, Tenzin Lhakhang, Donghui Li, Daniel J. Eichinger, David Fenyo and Jef D. Boeke

    Biological Procedures Online 2016 18:16

    Published on: 15 August 2016

  9. Research

    Isolation, expansion and characterization of porcine urinary bladder smooth muscle cells for tissue engineering

    A key requirements for therapy utilizing the tissue engineering methodologies is use of techniques which have the capability to yield a high number of cells, from small tissue biopsy in a relatively short time...

    Marta Pokrywczynska, Daria Balcerczyk, Arkadiusz Jundzill, Maciej Gagat, Monika Czapiewska, Tomasz Kloskowski, Maciej Nowacki, Agata M. Gastecka, Magdalena Bodnar, Alina Grzanka, Andrzej Marszalek and Tomasz Drewa

    Biological Procedures Online 2016 18:17

    Published on: 12 August 2016

  10. Methodology

    An optimised method for the proteomic profiling of full thickness human skin

    The skin is the largest organ of the human body and is the first line barrier defence against trauma, microbial infiltration and radiation. Skin diseases can be a result of multi-systemic disease or an isolate...

    Emily Bliss, Wendy E. Heywood, Malika Benatti, Neil J. Sebire and Kevin Mills

    Biological Procedures Online 2016 18:15

    Published on: 21 July 2016

  11. Methodology

    An Optimized Protocol for Packaging Pseudotyped Integrase Defective Lentivirus

    A number of integrase defective lentiviral (IDLV) packaging systems have been developed to produce integration deficient lentiviruses for gene delivery and epichromosomal expression. However, despite their gro...

    Ranjita Sengupta, Chandreyee Mukherjee, Nandita Sarkar, Zhihong Sun, Jacob Lesnik, Joseph Huang and Biao Lu

    Biological Procedures Online 2016 18:14

    Published on: 11 July 2016

  12. Methodology

    Reliable FASP-based procedures for optimal quantitative proteomic and phosphoproteomic analysis on samples from acute myeloid leukemia patients

    Satisfactory sample preparation for mass spectrometry-based analysis is a critical step in the proteomics workflow. The quality and reproducibility of sample preparation can determine the coverage and confiden...

    Maria Hernandez-Valladares, Elise Aasebø, Olav Mjaavatten, Marc Vaudel, Øystein Bruserud, Frode Berven and Frode Selheim

    Biological Procedures Online 2016 18:13

    Published on: 21 June 2016

  13. Short report

    A BioBrick™-Compatible Vector for Allelic Replacement Using the XylE Gene as Selection Marker

    Circular plasmid-mediated homologous recombination is commonly used for marker-less allelic replacement, exploiting the endogenous recombination machinery of the host. Common limitations of existing methods in...

    Michela Casanova, Lorenzo Pasotti, Susanna Zucca, Nicolò Politi, Ilaria Massaiu, Cinzia Calvio, Maria Gabriella Cusella De Angelis and Paolo Magni

    Biological Procedures Online 2016 18:6

    Published on: 13 February 2016

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