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  1. Content type: OriginalPaper

    The UBA domain is a conserved sequence motif among polyubiquitin binding proteins. For the first time, we demonstrate a systematic, high throughput approach to identification of UBA domain-interacting proteins...

    Authors: Julia W. Pridgeon, Thangiah Geetha and Marie W. Wooten

    Citation: Biological Procedures Online 2003 5:51228

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  2. Content type: OriginalPaper

    Integrin adhesion molecules have important adhesion and signaling functions. They also play a central role in the pathogenesis of many autoimmune diseases. Over the past few years we have described a T cell ad...

    Authors: Raymond L. Yung, Donna Ray, Ru Ran Mo and Jun Chen

    Citation: Biological Procedures Online 2003 5:51211

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  3. Content type: OriginalPaper

    The discovery of oncogenes and signal transduction pathways important for mitogenesis has triggered the development of target-specific small molecule anti-cancer compounds. As exemplified by imatinib (Gleevec)...

    Authors: Mohammad Azam, Tal Raz, Valentina Nardi, Sarah L. Opitz and George Q. Daley

    Citation: Biological Procedures Online 2003 5:51204

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  4. Content type: OriginalPaper

    Salmonella enterica serovar Typhimurium is capable of swarming over semi-solid surfaces. Although its swarming behavior shares many readily observable similarities with other swarming bacteria, t...

    Authors: Wook Kim and Michael G. Surette

    Citation: Biological Procedures Online 2003 5:51189

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  5. Content type: OriginalPaper

    The 72 kDa heat shock protein (HSP72) is a molecular chaperone that binds native protein with low affinity. These interactions can alter function of the substrate, a property known as HSP-mediated activity con...

    Authors: Xinsheng Zhang and Michael Oglesbee

    Citation: Biological Procedures Online 2003 5:51170

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  6. Content type: OriginalPaper

    Assembly of DNA into chromatin allows for the formation of a barrier that protects naked DNA from protein and chemical agents geared to degrade or metabolize DNA. Chromatin assembly occurs whenever a length of...

    Authors: Troy A. A. Harkness, Terra G. Arnason, Charmaine Legrand and Ashley Lone

    Citation: Biological Procedures Online 2003 5:51162

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  7. Content type: OriginalPaper

    Here we describe a detailed methodology to study the function of genes whose products function during mitosis by dsRNA-mediated interference (RNAi) in cultured cells ofDrosophila melanogaster. This procedure is p...

    Authors: Helder Maiato, Claudio E. Sunkel and William C. Earnshaw

    Citation: Biological Procedures Online 2003 5:51153

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  8. Content type: OriginalPaper

    We have developed two whole genome-scanning techniques to aid in the discovery of polymorphisms as well as horizontally acquired genes in prokaryotic organisms.First, two-dimensional bacterial genomic display (2D...

    Authors: Chad Malloff, Edie Dullaghan, Alice Li, Richard Stokes, Rachel Fernandez and Wan Lam

    Citation: Biological Procedures Online 2003 5:51143

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  9. Content type: OriginalPaper

    Here we describe in more depth the previously published application of the fluorescent probe 4,5-diaminofluorescein (DAF-2) in order to reliably measure low levels of nitric oxide (NO) as released from human e...

    Authors: Thomas R. Räthel, Jürgen F. Leikert, Angelika M. Vollmar and Verena M. Dirsch

    Citation: Biological Procedures Online 2003 5:51136

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  10. Content type: OriginalPaper

    Tetrahydromethanopterin (H4MPT) is a tetrahydrofolate analog originally discovered in methanogenic archaea, but later found in other archaea and bacteria. The extent to which H4MPT occurs among living organisms i...

    Authors: Matthew E. Bechard, Sonya Chhatwal, Rosemarie E. Garcia and Madeline E. Rasche

    Citation: Biological Procedures Online 2003 5:51069

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  11. Content type: OriginalPaper

    Satellite DNA sequences are known to be highly variable and to have been subjected to concerted evolution that homogenizes member sequences within species. We have analyzed the mode of evolution of satellite D...

    Authors: Mikio Kato

    Citation: Biological Procedures Online 2003 5:51063

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  12. Content type: OriginalPaper

    Type I interferon (IFN) gene therapy modulates the immune response leading to inflammatory heart disease following cytomegalovirus (CMV) infection in a murine model of post-viral myocarditis. Efficacy of diffe...

    Authors: Emmalene J. Bartlett, Vanessa S. Cull, Eva N. Mowe, Josephine P. Mansfield and Cassandra M. James

    Citation: Biological Procedures Online 2003 5:51043

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  13. Content type: OriginalPaper

    Relocation into the nucleus of the yeast cytoplasmic linear plasmids was studied using a monitor plasmid pCLU1. InSaccharomyces cerevisiae, the nuclearly-relocated pCLU1 replicated in a linear form (termed pTLU-t...

    Authors: Norio Gunge, Hideki Takata, Akira Matsuura and Kohsai Fukuda

    Citation: Biological Procedures Online 2003 5:51029

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  14. Content type: OriginalPaper

    Conjugated linoleic acid (CLA) can activate (in vitro) the nuclear transcription factors known as the peroxisome proliferators activated receptors (PPAR). CLA was fed at 11 g CLA/kg of feed for 45d to castrated m...

    Authors: W. J. Meadus

    Citation: Biological Procedures Online 2003 5:51020

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  15. Content type: OriginalPaper

    Disarmed versions of the cytotoxin ricin can deliver fused peptides into target cells leading to MHC class I-restricted antigen presentation [Smithet al. J Immunol 2002; 169:99–107]. The ricin delivery vector mus...

    Authors: Daniel C. Smith, Catherine J. Marsden, J. Michael Lord and Lynne M. Roberts

    Citation: Biological Procedures Online 2003 5:51013

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  16. Content type: OriginalPaper

    Real-time PCR methodology can successfully quantitate microchimeric cell populations at a concentration of 100 microchimeric cells/100,000 host cells; however, it has not been successful in quantitating DNA fr...

    Authors: Carol M. Artlett, C. Gennaro Dito and Paul J. Christner

    Citation: Biological Procedures Online 2003 5:10103

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  17. Content type: OriginalPaper

    It is widely acknowledged that the presence of extracellular matrix components as substrates can drastically modulate the phenotype and gene expression of cultured cells, including tumor cells. A number of pub...

    Authors: Rosalia Sirchia, Valentina Ciacciofera and Claudio Luparello

    Citation: Biological Procedures Online 2003 5:222

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  18. Content type: OriginalPaper

    Shotgun phage display cloning involves construction of libraries from randomly fragmented bacterial chromosomal DNA, cloned genes, or eukaryotic cDNAs, into a phagemid vector. The library obtained consists of ...

    Authors: Karin Jacobsson, Anna Rosander, Joakim Bjerketorp and Lars Frykberg

    Citation: Biological Procedures Online 2003 5:123

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  19. Content type: OriginalPaper

    Microsatellite markers are simple sequence repeats within the mammalian genome that can be used for identifying disease loci, mapping genes of interest as well as studying segregation patterns related to meiot...

    Authors: Lara A. Underkoffler, Joelle N. Collins, Jonathan D. Choi and Rebecca J. Oakey

    Citation: Biological Procedures Online 2003 5:116

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  20. Content type: OriginalPaper

    Excitable cells in many endocrine and neuronal systems display rhythms with periodicities on the order of many minutes. To observe firing patterns that represent the output of these rhythms requires a recordin...

    Authors: Craig S. Nunemaker, R. Anthony DeFazio and Suzanne M. Moenter

    Citation: Biological Procedures Online 2003 5:53

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  21. Content type: OriginalPaper

    There is accumulating evidence that membrane-bound receptors interact with many intracellular proteins. Multiprotein complexes associated with ionotropic receptors have been extensively characterized, but the ...

    Authors: Carine Bécamel, Nathalie Galéotti, Joël Poncet, Patrick Jouin, Aline Dumuis, Joël Bockaert and Philippe Marin

    Citation: Biological Procedures Online 2002 4:41094

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  22. Content type: OriginalPaper

    Human herpesvirus (HHV)-6B is a pathogen causing latent infection in virtually all humans. Nevertheless, the interaction of HHV-6B with its host cells is poorly understood. Although HHV-6B is approximately 90%...

    Authors: Bodil Øster and Per Höllsberg

    Citation: Biological Procedures Online 2002 4:41088

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  23. Content type: OriginalPaper

    The described method allows for detection of rare linear DNA fragments generated during genomic deletions. The predicted limit of the detection is one DNA molecule per 107 or more cells. The method is based on an...

    Authors: Sergei V. Saveliev

    Citation: Biological Procedures Online 2002 4:41070

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  24. Content type: OriginalPaper

    The cell cycle machinery consists of regulatory proteins that control the progression through the cell cycle ensuring that DNA replication alternates with DNA segregation in mitosis to maintain cell integrity....

    Authors: Andrea Klotzbucher, Gaetan Pascreau, Claude Prigent and Yannick Arlot-Bonnemains

    Citation: Biological Procedures Online 2002 4:41062

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  25. Content type: OriginalPaper

    Established cell lines are invaluable for studying cell and molecular biological questions. A variety of human ovarian cancer (OC) cell lines exist, however, most have acquired significant genetic alterations ...

    Authors: Lesley D. Dunfield, Trevor G. Shepherd and Mark W. Nachtigal

    Citation: Biological Procedures Online 2002 4:41055

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  26. Content type: OriginalPaper

    The “in vitro virus” is a molecular construct to perform evolutionary protein engineering. The “virion(=viral particle)”(mRNA-peptide fusion), is made by bonding a nascent protein with its coding mRNA via puromyc...

    Authors: Ichiro Tabuchi, Sayaka Soramoto, Miho Suzuki, Koichi Nishigaki, Naoto Nemoto and Yuzuru Husimi

    Citation: Biological Procedures Online 2002 4:41049

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  27. Content type: OriginalPaper

    Recent evidence indicates novel role for matrix metalloproteinases (MMPs), in particular gelatinase A (MMP-2), in the regulation of vascular biology that are unrelated to their well-known proteolytic breakdown...

    Authors: Carlos Fernandez-Patron, Christine Zouki, Randy M. Whittal, John S. D. Chan, Sandra T. Davidge and János G. Filep

    Citation: Biological Procedures Online 2002 4:41038

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  28. Content type: OriginalPaper

    We describe here a method for isolating endothelial cells from rat heart blood vessels by means of coronary microperfusion with collagenase. This method makes it possible to obtain high amounts of endothelial ...

    Authors: Silvia Nistri, Luca Mazzetti, Paola Failli and Daniele Bani

    Citation: Biological Procedures Online 2002 4:41032

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  29. Content type: OriginalPaper

    The study of hematopoietic stem cells (HSCs) and the process by which they differentiate into committed progenitors has been hampered by the lack of in vitro clonal assays that can support erythroid, myeloid and ...

    Authors: Marcus O. Muench, David L. Suskind and Alicia Bárcena

    Citation: Biological Procedures Online 2002 4:41010

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  30. Content type: OriginalPaper

    Angiogenesis, a key step in many physiological and pathological processes, involves proteolysis of the extracellular matrix. To study the role of two enzymatic families, serine-proteases and matrix metalloprot...

    Authors: Véronique Masson, Laetitia Devy, Christine Grignet-Debrus, Sarah Bernt, Khalid Bajou, Silvia Blacher, Guy Roland, Yawen Chang, Timothy Fong, Peter Carmeliet, Jean-Michel Foidart and Agnès Noël

    Citation: Biological Procedures Online 2002 4:24

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  31. Content type: OriginalPaper

    A number of factors influence the development of tolerance, including the nature, concentration and mode of antigen presentation to the immune system, as well as the age of the host. The studies were conducted...

    Authors: Ke-Qin Xin, Shin Sasaki, Yoshitsugu Kojima, Nao Jounai, Yasuko Kumamoto, Kumiko Hashimoto, Kaori Shinoda, Kenji Hamajima and Kenji Okuda

    Citation: Biological Procedures Online 2001 3:31091

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  32. Content type: OriginalPaper

    The promoter activity of the rat Ca2+/calmodulin-dependent protein kinase II gene was analyzed using the luciferase reporter gene in neuronal and non-neuronal cell lines. Neuronal cell type-specific promoter acti...

    Authors: Kazuko Mima, Hitomi Donai and Takashi Yamauchi

    Citation: Biological Procedures Online 2001 3:31079

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  33. Content type: OriginalPaper

    Gene expression and regulation are mediated by DNA sequences, in most instances, directly upstream to the coding sequences by recruiting transcription factors, regulators, and a RNA polymerase in a spatially d...

    Authors: Nitin S. Baliga

    Citation: Biological Procedures Online 2001 3:31064

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  34. Content type: OriginalPaper

    In platelets, PGHS-1-dependant formation of thromboxane A2 is an important modulator of platelet function and a target for pharmacological inhibition of platelet function by aspirin. Since platelets are anucleate...

    Authors: Douglas J. Franks, Cameron Mroske and Odette Laneuville

    Citation: Biological Procedures Online 2001 3:31054

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  35. Content type: OriginalPaper

    Isolated adult cardiac ventricular myocytes have been a useful model for cardiovascular research for more than 20 years. With the recent advances in cellular physiology and transgenic techniques, direct measur...

    Authors: Jun Ren and Loren E. Wold

    Citation: Biological Procedures Online 2001 3:31043

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  36. Content type: OriginalPaper

    Mammalian sperm acrosomes contain several glycohydrolases that are thought to aid in the dispersion and digestion of vestments surrounding the egg. In this study, we have used multiple approaches to examine th...

    Authors: Aida Abou-Haila and Daulat R. P. Tulsiani

    Citation: Biological Procedures Online 2001 3:31035

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  37. Content type: OriginalPaper

    We describe a semiquantitative RT-PCR protocol optimized in our laboratory to extract RNA from as little as 10,000 cells and to measure the expression levels of several target mRNAs from each sample. This proc...

    Authors: Maria Marone, Simona Mozzetti, Daniela De Ritis, Luca Pierelli and Giovanni Scambia

    Citation: Biological Procedures Online 2001 3:31019

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  38. Content type: OriginalPaper

    The study of parasitic protozoa plays a major role in cell biology, biochemistry and molecular biology. Numerous cytochemical techniques have been developed in order to unequivocally identify the nature of sub...

    Authors: Marcos A. Vannier-Santos and Ulysses Lins

    Citation: Biological Procedures Online 2001 3:31008

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  39. Content type: OriginalPaper

    We established a sensitive non-radioactive in situ hybridization (ISH) method for the detection of chicken IgG γ-chain mRNA in paraffin sections. RNA probes were transcribed in vitro fromcloned chicken IgG CH1 nu...

    Authors: Weiming Zheng, Junichi Izaki, Shuichi Furusawa and Yukinori Yoshimura

    Citation: Biological Procedures Online 2001 3:31001

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  40. Content type: OriginalPaper

    The oxidative modification of proteins has been shown to play a major role in a number of human diseases. However, the ability to identify specific proteins that are most susceptible to oxidative modifications...

    Authors: Craig C. Conrad, John M. Talent, Christina A. Malakowsky and Robert W. Gracy

    Citation: Biological Procedures Online 1999 2:210039

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  41. Content type: OriginalPaper

    Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financi...

    Authors: Wim Van Criekinge and Rudi Beyaert

    Citation: Biological Procedures Online 1999 2:210001

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  42. Content type: OriginalPaper

    It had been suggested that influenza virus-mediated membrane fusion might be dependent on a pH gradient across a target membrane. We have designed experiments in which this issue could be addressed. Two popula...

    Authors: João Ramalho-Santos and Maria C. Pedroso de Lima

    Citation: Biological Procedures Online 1999 1:13107

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