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  1. DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method ...

    Authors: Fatma Mseddi, Mohammed Ali Jarboui, Amira Sellami, Hayet Sellami and Ali Ayadi

    Citation: Biological Procedures Online 2011 13:5

    Content type: Research

    Published on:

  2. We describe a simple and reproducible method to measure absolute telomere length (aTL) using quantitative real-time polymerase chain reaction (qPCR). This method is based on the Cawthon method for relative mea...

    Authors: Nathan J O'Callaghan and Michael Fenech

    Citation: Biological Procedures Online 2011 13:3

    Content type: Methodology

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  3. Real-time quantitative RT-PCR (RT-qPCR) is a “gold” standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight indi...

    Authors: Gang Chen, Peter Kronenberger, Erik Teugels and Jacques De Grève

    Citation: Biological Procedures Online 2010 17:9034

    Content type: OriginalPaper

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  4. Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight indi...

    Authors: Gang Chen, Peter Kronenberger, Erik Teugels and Jacques De Grève

    Citation: Biological Procedures Online 2010 13:1

    Content type: Research

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  5. NF-κB regulates several important expressions, such as cytokine release, anti-apoptosis, adhesion molecule expression, and cell cycle processing. Several NF-κB inhibitors have been discovered as an anti-tumor ...

    Authors: Yu-Ling Lin, Yen-Shun Chen, Jui-Hung Hsieh, Ching-Min Lin, Hsin-Yi Wu, Chen-Si Lin, Rea-Min Chu, Kuang-Wen Liao and Yuan-Hsun Hsu

    Citation: Biological Procedures Online 2010 12:131

    Content type: Methodology

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  6. The use of architectural and morphological characteristics of cells for establishing prognostic indicators by which individual pathologies are assigned grade and stage is a well-accepted practice. Advances in ...

    Authors: John N McGinley, Matthew D Thompson and Henry J Thompson

    Citation: Biological Procedures Online 2010 12:118

    Content type: Methodology

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  7. There are several traditional and well-developed methods for analyzing the activity of transcription factors, such as EMSA, enzyme-linked immunosorbent assay, and reporter gene activity assays. All of these me...

    Authors: Yu-Ling Lin, Yun-Ju Lai, Nu-Man Tsai, Tai-Chu Peng, Yen-Ku Liu, Ru-Ping Lee, Chueh-Jen Tsai and Kuang-Wen Liao

    Citation: Biological Procedures Online 2010 12:18

    Content type: Methodology

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  8. Development of antimitotic binding to the colchicine-binding site for the treatment of cancer is rapidly expanding. Numerous antimicrotubule agents are prepared every year, and the determination of their bindi...

    Authors: Sébastien Fortin, Jacques Lacroix, Marie-France Côté, Emmanuel Moreau, Éric Petitclerc and René C-Gaudreault

    Citation: Biological Procedures Online 2010 12:113

    Content type: Methodology

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  9. Indoleamine 2,3 dioxygenase (IDO) is a potent immunomodulatory enzyme that has recently attracted significant attention for its potential application as an inducer of immunotolerance in transplantation. We hav...

    Authors: Alireza Moeen Rezakhanlou, Darya Habibi, Amy Lai, Reza B Jalili, Christopher J Ong and Aziz Ghahary

    Citation: Biological Procedures Online 2010 12:107

    Content type: Research

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  10. Northern blot hybridization is a useful tool for analyzing transcript patterns. To get a picture of what really occurs in vivo, it is necessary to use a protocol allowing full protection of the RNA integrity a...

    Authors: Marcello Tagliavia, Anna Taravella, Sandra Marineo, Anna Maria Puglia and Mario La Farina

    Citation: Biological Procedures Online 2010 12:27

    Content type: Methodology

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  11. Laser capture microdissection (LCM) enables collection of cell populations highly enriched for specific cell types that have the potential of yielding critical information about physiological and pathophysiolo...

    Authors: John N McGinley, Zongjian Zhu, Weiqin Jiang and Henry J Thompson

    Citation: Biological Procedures Online 2010 12:31

    Content type: Research

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  12. The aim of this study was to compare different cell sources and culture conditions to obtain endothelial progenitor cells (EPCs) with predictable antigen pattern, proliferation potential and in vitro vasculoge...

    Authors: Claudio Muscari, Chiara Gamberini, Ilaria Basile, Francesca Bonafé, Simond Valgimigli, Ombretta Capitani, Carlo Guarnieri and Claudio Marcello Caldarera

    Citation: Biological Procedures Online 2010 12:89

    Content type: Research

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  13. The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide...

    Authors: Nakul Mandal, Steffen Heegaard, Jan Ulrik Prause, Bent Honoré and Henrik Vorum

    Citation: Biological Procedures Online 2009 12:56

    Content type: Review

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  14. Subtraction technique has been broadly applied for target gene discovery. However, most current protocols apply relative differential subtraction and result in great amount clone mixtures of unique and differe...

    Authors: Ning Wu, Kanyand Matand and Sonya Williams

    Citation: Biological Procedures Online 2009 12:44

    Content type: Methodology

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  15. Analgesic and anesthetic drugs may have an impact on the results achieved from animal experiments. In the study presented here, we try to enlighten whether anesthesia with fentanyl/fluniasone and midazolam (Hy...

    Authors: Anita Gothelf, Pernille Hojman and Julie Gehl

    Citation: Biological Procedures Online 2009 11:325

    Content type: Research

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  16. Rottlerin is a natural product isolated from Mallotus philippinensis. This polyphenolic compound, originally described as a selective inhibitor of PKCδ, can inhibit many other PKC-unrelated kinases and has a numb...

    Authors: Emanuela Maioli, Claudia Torricelli, Vittoria Fortino, Filippo Carlucci, Valentina Tommassini and Adriana Pacini

    Citation: Biological Procedures Online 2009 11:227

    Content type: Research

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  17. Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas...

    Authors: Jeffrey D Carter, Stacey B Dula, Kathryn L Corbin, Runpei Wu and Craig S Nunemaker

    Citation: Biological Procedures Online 2009 11:3

    Content type: Review

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  18. The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of serious pathological states such as cancer and fibrosis. In t...

    Authors: Andrea Staršíchová, Lukáš Kubala, Eva Lincová, Zuzana Pernicová, Alois Kozubík and Karel Souček

    Citation: Biological Procedures Online 2009 11:316

    Content type: Methodology

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  19. MicroRNAs are positive and negative regulators of eukaryotic gene expression that modulate transcript abundance by specific binding to sequence motifs located prevalently in the 3' untranslated regions of targ...

    Authors: Fredy Siegrist, Thomas Singer and Ulrich Certa

    Citation: Biological Procedures Online 2009 11:113

    Content type: Research

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  20. In the image segmentation process of positron emission tomography combined with computed tomography (PET/CT) imaging, previous works used information in CT only for segmenting the image without utilizing the i...

    Authors: Iman Avazpour, M Iqbal Saripan, Abdul Jalil Nordin and Rajaa Syamsul Azmir Iman Abdullah

    Citation: Biological Procedures Online 2009 11:241

    Content type: Methodology

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  21. Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effecti...

    Authors: Adria D Dismuke, Aimee D Kohn, Randall T Moon and Melissa H Wong

    Citation: Biological Procedures Online 2009 11:130

    Content type: Research

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  22. Many species of streptococci secrete and use a competence-stimulating peptide (CSP) to initiate quorum sensing for induction of genetic competence, bacteriocin production, and other activities. These signaling...

    Authors: XiaoLin Tian, Raymond T Syvitski, TianLei Liu, Nadine Livingstone, David L Jakeman and Yung-Hua Li

    Citation: Biological Procedures Online 2009 11:207

    Content type: Methodology

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  23. Evaluation of blood smear is a commonly clinical test these days. Most of the time, the hematologists are interested on white blood cells (WBCs) only. Digital image processing techniques can help them in their...

    Authors: Farnoosh Sadeghian, Zainina Seman, Abdul Rahman Ramli, Badrul Hisham Abdul Kahar and M-Iqbal Saripan

    Citation: Biological Procedures Online 2009 11:196

    Content type: Methodology

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  24. Comparative genomics has become a real tantalizing challenge in the postgenomic era. This fact has been mostly magnified by the plethora of new genomes becoming available in a daily bases. The overwhelming lis...

    Authors: Jennifer Commins, Christina Toft and Mario A Fares

    Citation: Biological Procedures Online 2009 11:52

    Content type: Review

    Published on:

  25. The dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with enzyme-fre...

    Authors: Boon C Heng, Catherine M Cowan and Shubhayu Basu

    Citation: Biological Procedures Online 2009 11:161

    Content type: Research

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  26. Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for...

    Authors: Alina Rodríguez-Mallon, Yamilet Cárdenas, Juana María Lugo, Aymé Oliva, Antonio Morales and Mario Pablo Estrada

    Citation: Biological Procedures Online 2009 11:79

    Content type: Methodology

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  27. As many as 50% of adults with type I (T1) diabetes exhibit bone loss and are at increased risk for fractures. Therapeutic development to prevent bone loss and/or restore lost bone in T1 diabetic patients requi...

    Authors: Katherine Motyl and Laura R McCabe

    Citation: Biological Procedures Online 2009 11:296

    Content type: Research

    Published on:

  28. Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the complex organization of the mammalian testicular tissue, var...

    Authors: Rosana Rodríguez-Casuriaga, Adriana Geisinger, Beatriz López-Carro, Valentina Porro, Rodolfo Wettstein and Gustavo A Folle

    Citation: Biological Procedures Online 2009 11:184

    Content type: Methodology

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  29. The present study describes a protocol to generate heterogenous populations of neurotransmitter-producing neurons from human mesenchymal stem cells (MSCs). MSCs are bone marrow (BM)-derived cells which undergo...

    Authors: Steven J. Greco, Chunyi Zhou, Jiang-Hong Ye and Pranela Rameshwar

    Citation: Biological Procedures Online 2008 10:101000090

    Content type: OriginalPaper

    Published on:

  30. The early chick embryo contains subpopulations of cells that express lineage-specific transcription factors. We have developed protocols to examine the role of these cells during development that involve label...

    Authors: Jacquelyn Gerhart, Christine Neely, Jessica Pfautz and Mindy George-Weinstein

    Citation: Biological Procedures Online 2008 10:101000074

    Content type: OriginalPaper

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  31. Gene families are widely used in comparative genomics, molecular evolution, and in systematics. However, they are constructed in different manners, their data analyzed and interpreted differently, with differe...

    Authors: David A. Liberles and Katharina Dittmar

    Citation: Biological Procedures Online 2008 10:101066

    Content type: OriginalPaper

    Published on:

  32. DNA methyltransferase 1 (DNMT1) is the enzyme responsible for the maintenance of DNA methylation patterns during cell division. DNMT1 expression is tightly regulated within the cell cycle. Our previous study s...

    Authors: Alexander Unterberger, Jérôme Torrisani and Moshe Szyf

    Citation: Biological Procedures Online 2008 10:101047

    Content type: OriginalPaper

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  33. Acetyl phosphate (acetyl-P) serves critical roles in coenzyme A recycling and ATP synthesis. It is the intermediate of the Pta-AckA pathway that inter-converts acetyl-coenzyme A and acetate. Acetyl-P also can ...

    Authors: David H. Keating, Ana Shulla, Adam H. Klein and Alan J. Wolfe

    Citation: Biological Procedures Online 2008 10:101036

    Content type: OriginalPaper

    Published on:

  34. Calcitriol (1α, 25-dihydroxyvitamin D3) induces the expression of CD14 in mononuclear phagocytes. The mechanisms accounting for this have been unclear since the promoter of CD14 does not contain a canonical vitam...

    Authors: Alireza Moeenrezakhanlou, Devki Nandan and Neil E. Reiner

    Citation: Biological Procedures Online 2008 10:101029

    Content type: OriginalPaper

    Published on:

  35. We developed a rapid mutagenesis method based on a modification of the QuikChange® system (Stratagene) to systemically replace endogenous gene sequences with a unique similar size sequence tag. The modificatio...

    Authors: Qiwen Deng, Wensheng Luo and Michael S. Donnenberg

    Citation: Biological Procedures Online 2007 9:91018

    Content type: OriginalPaper

    Published on:

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