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  1. The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide...

    Authors: Nakul Mandal, Steffen Heegaard, Jan Ulrik Prause, Bent Honoré and Henrik Vorum

    Citation: Biological Procedures Online 2009 12:56

    Content type: Review

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  2. Subtraction technique has been broadly applied for target gene discovery. However, most current protocols apply relative differential subtraction and result in great amount clone mixtures of unique and differe...

    Authors: Ning Wu, Kanyand Matand and Sonya Williams

    Citation: Biological Procedures Online 2009 12:44

    Content type: Methodology

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  3. Analgesic and anesthetic drugs may have an impact on the results achieved from animal experiments. In the study presented here, we try to enlighten whether anesthesia with fentanyl/fluniasone and midazolam (Hy...

    Authors: Anita Gothelf, Pernille Hojman and Julie Gehl

    Citation: Biological Procedures Online 2009 11:325

    Content type: Research

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  4. Rottlerin is a natural product isolated from Mallotus philippinensis. This polyphenolic compound, originally described as a selective inhibitor of PKCδ, can inhibit many other PKC-unrelated kinases and has a numb...

    Authors: Emanuela Maioli, Claudia Torricelli, Vittoria Fortino, Filippo Carlucci, Valentina Tommassini and Adriana Pacini

    Citation: Biological Procedures Online 2009 11:227

    Content type: Research

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  5. Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas...

    Authors: Jeffrey D Carter, Stacey B Dula, Kathryn L Corbin, Runpei Wu and Craig S Nunemaker

    Citation: Biological Procedures Online 2009 11:3

    Content type: Review

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  6. The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of serious pathological states such as cancer and fibrosis. In t...

    Authors: Andrea Staršíchová, Lukáš Kubala, Eva Lincová, Zuzana Pernicová, Alois Kozubík and Karel Souček

    Citation: Biological Procedures Online 2009 11:316

    Content type: Methodology

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  7. MicroRNAs are positive and negative regulators of eukaryotic gene expression that modulate transcript abundance by specific binding to sequence motifs located prevalently in the 3' untranslated regions of targ...

    Authors: Fredy Siegrist, Thomas Singer and Ulrich Certa

    Citation: Biological Procedures Online 2009 11:113

    Content type: Research

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  8. In the image segmentation process of positron emission tomography combined with computed tomography (PET/CT) imaging, previous works used information in CT only for segmenting the image without utilizing the i...

    Authors: Iman Avazpour, M Iqbal Saripan, Abdul Jalil Nordin and Rajaa Syamsul Azmir Iman Abdullah

    Citation: Biological Procedures Online 2009 11:241

    Content type: Methodology

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  9. Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effecti...

    Authors: Adria D Dismuke, Aimee D Kohn, Randall T Moon and Melissa H Wong

    Citation: Biological Procedures Online 2009 11:130

    Content type: Research

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  10. Many species of streptococci secrete and use a competence-stimulating peptide (CSP) to initiate quorum sensing for induction of genetic competence, bacteriocin production, and other activities. These signaling...

    Authors: XiaoLin Tian, Raymond T Syvitski, TianLei Liu, Nadine Livingstone, David L Jakeman and Yung-Hua Li

    Citation: Biological Procedures Online 2009 11:207

    Content type: Methodology

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  11. Evaluation of blood smear is a commonly clinical test these days. Most of the time, the hematologists are interested on white blood cells (WBCs) only. Digital image processing techniques can help them in their...

    Authors: Farnoosh Sadeghian, Zainina Seman, Abdul Rahman Ramli, Badrul Hisham Abdul Kahar and M-Iqbal Saripan

    Citation: Biological Procedures Online 2009 11:196

    Content type: Methodology

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  12. Comparative genomics has become a real tantalizing challenge in the postgenomic era. This fact has been mostly magnified by the plethora of new genomes becoming available in a daily bases. The overwhelming lis...

    Authors: Jennifer Commins, Christina Toft and Mario A Fares

    Citation: Biological Procedures Online 2009 11:52

    Content type: Review

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  13. The dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with enzyme-fre...

    Authors: Boon C Heng, Catherine M Cowan and Shubhayu Basu

    Citation: Biological Procedures Online 2009 11:161

    Content type: Research

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  14. Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for...

    Authors: Alina Rodríguez-Mallon, Yamilet Cárdenas, Juana María Lugo, Aymé Oliva, Antonio Morales and Mario Pablo Estrada

    Citation: Biological Procedures Online 2009 11:79

    Content type: Methodology

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  15. As many as 50% of adults with type I (T1) diabetes exhibit bone loss and are at increased risk for fractures. Therapeutic development to prevent bone loss and/or restore lost bone in T1 diabetic patients requi...

    Authors: Katherine Motyl and Laura R McCabe

    Citation: Biological Procedures Online 2009 11:296

    Content type: Research

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  16. Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the complex organization of the mammalian testicular tissue, var...

    Authors: Rosana Rodríguez-Casuriaga, Adriana Geisinger, Beatriz López-Carro, Valentina Porro, Rodolfo Wettstein and Gustavo A Folle

    Citation: Biological Procedures Online 2009 11:184

    Content type: Methodology

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  17. The present study describes a protocol to generate heterogenous populations of neurotransmitter-producing neurons from human mesenchymal stem cells (MSCs). MSCs are bone marrow (BM)-derived cells which undergo...

    Authors: Steven J. Greco, Chunyi Zhou, Jiang-Hong Ye and Pranela Rameshwar

    Citation: Biological Procedures Online 2008 10:101000090

    Content type: OriginalPaper

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  18. The early chick embryo contains subpopulations of cells that express lineage-specific transcription factors. We have developed protocols to examine the role of these cells during development that involve label...

    Authors: Jacquelyn Gerhart, Christine Neely, Jessica Pfautz and Mindy George-Weinstein

    Citation: Biological Procedures Online 2008 10:101000074

    Content type: OriginalPaper

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  19. Gene families are widely used in comparative genomics, molecular evolution, and in systematics. However, they are constructed in different manners, their data analyzed and interpreted differently, with differe...

    Authors: David A. Liberles and Katharina Dittmar

    Citation: Biological Procedures Online 2008 10:101066

    Content type: OriginalPaper

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  20. DNA methyltransferase 1 (DNMT1) is the enzyme responsible for the maintenance of DNA methylation patterns during cell division. DNMT1 expression is tightly regulated within the cell cycle. Our previous study s...

    Authors: Alexander Unterberger, Jérôme Torrisani and Moshe Szyf

    Citation: Biological Procedures Online 2008 10:101047

    Content type: OriginalPaper

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  21. Acetyl phosphate (acetyl-P) serves critical roles in coenzyme A recycling and ATP synthesis. It is the intermediate of the Pta-AckA pathway that inter-converts acetyl-coenzyme A and acetate. Acetyl-P also can ...

    Authors: David H. Keating, Ana Shulla, Adam H. Klein and Alan J. Wolfe

    Citation: Biological Procedures Online 2008 10:101036

    Content type: OriginalPaper

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  22. Calcitriol (1α, 25-dihydroxyvitamin D3) induces the expression of CD14 in mononuclear phagocytes. The mechanisms accounting for this have been unclear since the promoter of CD14 does not contain a canonical vitam...

    Authors: Alireza Moeenrezakhanlou, Devki Nandan and Neil E. Reiner

    Citation: Biological Procedures Online 2008 10:101029

    Content type: OriginalPaper

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  23. Understanding mechanisms of gene regulation has broad therapeutic implications for human disease. Here we describe a novel method for generating human cell lines that serve as reporters of transcriptional acti...

    Authors: Sandra L. Fernandez, David W. Russell and Peter J. Hurlin

    Citation: Biological Procedures Online 2007 9:91084

    Content type: OriginalPaper

    Published on:

  24. B cell subpopulations in the spleen have been extensively characterized phenotypically; however, biochemical properties of these cell populations following B cell antigen receptor engagement have not been full...

    Authors: Pierre Antony, Kristen Hoek, Bhaskarjyoti Sarmah and Wasif N. Khan

    Citation: Biological Procedures Online 2007 9:91073

    Content type: OriginalPaper

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  25. Recent advances in redox proteomics have provided significant insight into the role of oxidative modifications in cellular signalling and metabolism. At present, these techniques rely heavily on Western blots ...

    Authors: H. Fai Poon, Laila Abdullah, Jon Reed, Sarah M. Doore, Cyndi Laird, Venkat Mathura, Michael Mullan and Fiona Crawford

    Citation: Biological Procedures Online 2007 9:91065

    Content type: OriginalPaper

    Published on:

  26. In adults, hematopoiesis occurs in bone marrow (BM) through a complex process with differentiation of hematopoietic stem cells (HSCs) to immune and blood cells. Human HSCs and their progenitors express CD34. M...

    Authors: Nitixa Patel, Marianne Castillo and Pranela Rameshwar

    Citation: Biological Procedures Online 2007 9:91056

    Content type: OriginalPaper

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  27. We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cass...

    Authors: Seok Hwee Koo, Tan Ching Ong, Kok Ting Chong, Caroline Guat Lay Lee, Fook Tim Chew and Edmund Jon Deoon Lee

    Citation: Biological Procedures Online 2007 9:91027

    Content type: OriginalPaper

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  28. We developed a rapid mutagenesis method based on a modification of the QuikChange® system (Stratagene) to systemically replace endogenous gene sequences with a unique similar size sequence tag. The modificatio...

    Authors: Qiwen Deng, Wensheng Luo and Michael S. Donnenberg

    Citation: Biological Procedures Online 2007 9:91018

    Content type: OriginalPaper

    Published on:

  29. Suppression of gene expression by small interfering RNA (siRNA) has proved to be a gene-specific and cost effective alternative to other gene suppression technologies. Short hairpin RNAs (shRNAs) generated fro...

    Authors: Hailong Wu, Anh Dinh and Yin-Yuan Mo

    Citation: Biological Procedures Online 2007 9:91009

    Content type: OriginalPaper

    Published on:

  30. We have developed a system to visualize functionally activated neurons and their projections in the brain. This system utilizes a transgenic mouse, fos-tau-lacZ (FTL), which expresses the marker gene, lacZ, in ne...

    Authors: Mark Murphy, Ursula Greferath and Yvette M. Wilson

    Citation: Biological Procedures Online 2007 9:91001

    Content type: OriginalPaper

    Published on:

  31. Teleost gut associated lymphoid tissue (GALT) consists of leucocyte populations located both intraepithelially and in the lamina propria with no structural organization. The present study aims to assess differ...

    Authors: Irene Salinas, José Meseguer and Maria Ángeles Esteban

    Citation: Biological Procedures Online 2007 9:43

    Content type: OriginalPaper

    Published on:

  32. This paper was originally published in Biological Procedures Online (BPO) on March 23, 2006. It was brought to the attention of the journal and authors that reference 74 was incorrect. The original citation for r...

    Authors: M. B. I. Reaz, M. S. Hussain and F. Mohd-Yasin

    Citation: Biological Procedures Online 2006 8:81163

    Content type: OriginalPaper

    Published on:

  33. In this work we describe protocols for the generation of gene deletions and gene replacements using a temperature sensitive plasmid in Escherichia coli O157:H7. This technology requires flanking DNA to be cloned ...

    Authors: James R. Emmerson, David L. Gally and Andrew J. Roe

    Citation: Biological Procedures Online 2006 8:81153

    Content type: OriginalPaper

    Published on:

  34. Live cell fluorescence microscopy using fluorescent protein tags derived from jellyfish and coral species has been a successful tool to image proteins and dynamics in many species. Multi-colored aequorea fluoresc...

    Authors: Jianrun Xia, Song Hon H. Kim, Susan Macmillan and Ray Truant

    Citation: Biological Procedures Online 2006 8:81063

    Content type: OriginalPaper

    Published on:

  35. The application of naked DNA containing type I interferon (IFN) transgenes is a promising potential therapeutic approach for controlling chronic viral infections. Herein, we detail the application of this appr...

    Authors: Bobbie Ann Austin, Cassandra M. James, Peter Härle and Daniel J. J. Carr

    Citation: Biological Procedures Online 2006 8:81055

    Content type: OriginalPaper

    Published on:

  36. Microtubule inhibitors such as Vinblastine and Paclitaxel are chemotherapy agents that activate the mitotic spindle checkpoint, arresting cells in mitosis and leading to cell death. The pathways that connect m...

    Authors: Hiroshi Y. Yamada and Gary J. Gorbsky

    Citation: Biological Procedures Online 2006 8:81036

    Content type: OriginalPaper

    Published on:

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