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  1. A number of factors influence the development of tolerance, including the nature, concentration and mode of antigen presentation to the immune system, as well as the age of the host. The studies were conducted...

    Authors: Ke-Qin Xin, Shin Sasaki, Yoshitsugu Kojima, Nao Jounai, Yasuko Kumamoto, Kumiko Hashimoto, Kaori Shinoda, Kenji Hamajima and Kenji Okuda
    Citation: Biological Procedures Online 2001 3:31091
  2. The promoter activity of the rat Ca2+/calmodulin-dependent protein kinase II gene was analyzed using the luciferase reporter gene in neuronal and non-neuronal cell lines. Neuronal cell type-specific promoter acti...

    Authors: Kazuko Mima, Hitomi Donai and Takashi Yamauchi
    Citation: Biological Procedures Online 2001 3:31079
  3. Gene expression and regulation are mediated by DNA sequences, in most instances, directly upstream to the coding sequences by recruiting transcription factors, regulators, and a RNA polymerase in a spatially d...

    Authors: Nitin S. Baliga
    Citation: Biological Procedures Online 2001 3:31064
  4. In platelets, PGHS-1-dependant formation of thromboxane A2 is an important modulator of platelet function and a target for pharmacological inhibition of platelet function by aspirin. Since platelets are anucleate...

    Authors: Douglas J. Franks, Cameron Mroske and Odette Laneuville
    Citation: Biological Procedures Online 2001 3:31054
  5. Isolated adult cardiac ventricular myocytes have been a useful model for cardiovascular research for more than 20 years. With the recent advances in cellular physiology and transgenic techniques, direct measur...

    Authors: Jun Ren and Loren E. Wold
    Citation: Biological Procedures Online 2001 3:31043
  6. Mammalian sperm acrosomes contain several glycohydrolases that are thought to aid in the dispersion and digestion of vestments surrounding the egg. In this study, we have used multiple approaches to examine th...

    Authors: Aida Abou-Haila and Daulat R. P. Tulsiani
    Citation: Biological Procedures Online 2001 3:31035
  7. We describe a semiquantitative RT-PCR protocol optimized in our laboratory to extract RNA from as little as 10,000 cells and to measure the expression levels of several target mRNAs from each sample. This proc...

    Authors: Maria Marone, Simona Mozzetti, Daniela De Ritis, Luca Pierelli and Giovanni Scambia
    Citation: Biological Procedures Online 2001 3:31019
  8. The study of parasitic protozoa plays a major role in cell biology, biochemistry and molecular biology. Numerous cytochemical techniques have been developed in order to unequivocally identify the nature of sub...

    Authors: Marcos A. Vannier-Santos and Ulysses Lins
    Citation: Biological Procedures Online 2001 3:31008
  9. We established a sensitive non-radioactive in situ hybridization (ISH) method for the detection of chicken IgG γ-chain mRNA in paraffin sections. RNA probes were transcribed in vitro fromcloned chicken IgG CH1 nu...

    Authors: Weiming Zheng, Junichi Izaki, Shuichi Furusawa and Yukinori Yoshimura
    Citation: Biological Procedures Online 2001 3:31001
  10. The oxidative modification of proteins has been shown to play a major role in a number of human diseases. However, the ability to identify specific proteins that are most susceptible to oxidative modifications...

    Authors: Craig C. Conrad, John M. Talent, Christina A. Malakowsky and Robert W. Gracy
    Citation: Biological Procedures Online 1999 2:210039
  11. Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financi...

    Authors: Wim Van Criekinge and Rudi Beyaert
    Citation: Biological Procedures Online 1999 2:210001
  12. In this paper, we present our method for the measurement of contact angles on the surface of minerals during the bioleaching process because the standard deviation obtained in our measurements achieved unexpec...

    Authors: Aleksandra Skłodowska, Marek Woźniak and Renata Matlakowska
    Citation: Biological Procedures Online 1999 1:13114
  13. It had been suggested that influenza virus-mediated membrane fusion might be dependent on a pH gradient across a target membrane. We have designed experiments in which this issue could be addressed. Two popula...

    Authors: João Ramalho-Santos and Maria C. Pedroso de Lima
    Citation: Biological Procedures Online 1999 1:13107
  14. Stripping voltammetry (SV) is used to quantitatively determine concentrations of the anti-neoplastic drug mitomycin C (MMC) alone and in mixtures with 5-fluorouracil and cisplatin, both of which are used in co...

    Authors: Dolores Marín, Pedro Pérez, Carmen Teijeiro and Emil Paleček
    Citation: Biological Procedures Online 1998 1:120100
  15. A general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. Since the tyrosine residue in...

    Authors: Jae Il Lee, Seung Kyoon Woo, Keun Il Kim, Kyung Chan Park, Sung Hee Baek, Yung Joon Yoo and Chin Ha Chung
    Citation: Biological Procedures Online 1998 1:110092
  16. Planar lipid bilayers (PLB) were prepared by the Montal-Mueller technique in a FRAP system designed to simultaneously measure conductivity across, and lateral diffusion of, the bilayer. In the first stage of t...

    Authors: H. Duclohier, O. Helluin, E. Lea, A. R. Mackie and S. Ladha
    Citation: Biological Procedures Online 1998 1:110081
  17. The chemical modification of synthetic oligonucleotides has recently been investigated to improve their pharmacological utilization. In addition to chemical alterations of the backbone and of the heterocyclic ...

    Authors: G. M. Bonora, S. Zaramella and F. M. Veronese
    Citation: Biological Procedures Online 1998 1:110059
  18. Amplification of DNA from soil is often inhibited by co-purified contaminants. A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to va...

    Authors: C. Yeates, M. R. Gillings, A. D. Davison, N. Altavilla and D. A. Veal
    Citation: Biological Procedures Online 1998 1:110040
  19. The intestinal absorption of analgesic peptides (leucine enkephalin and kyotorphin) and modified peptides in rat were studied. Although these peptides were not absorbed, the absorbability (absorption clearance...

    Authors: Takashi Mizuma and Shoji Awazu
    Citation: Biological Procedures Online 1998 1:110032

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