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Construction of a bacterial autoinducer detection system in mammalian cells


Quorum sensing (QS) is a cell density-dependent signaling system used by bacteria to coordinate gene expression within a population. QS systems in Gram negative bacteria consist of transcription factors of the LuxR family and their acyl homoserine lactone (AHL) ligands. We describe here a method for examining QS signaling systems in mammalian cells that uses engineered LuxR-type proteins from the opportunistic pathogen, Pseudomonas aeruginosa, which can function as AHL-dependent transcription factors. The engineered proteins respond to their cognate ligands and display sequence specific DNA binding properties. This system has several potential biotechnological and biological applications. It may be used to characterize any LuxR-type protein, screen animal and plant cell extracts or exudates for compounds that mimic or interfere with AHL signaling or to screen different cell types for AHL inactivating activities.



quorum sensing


acylhomoserine lactone


N-3-oxodocanoyl-homoserine lactone


N-butyryl homoserine lactone


N-3-oxoheptanoyl homoserine lactone


protein module containing three copies of the VP16 transcriptional activation domain (TAD) and the nuclear localization signal (NLS) from SV40 T antigen




Simple Modular Architecture Research Tool


autoinducer binding domain


helix-turn-helix DNA binding domain


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Correspondence to Kendra P. Rumbaugh Ph.D..

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Published: December 30, 2004.

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Shiner, E.K., Reddy, S., Timmons, C. et al. Construction of a bacterial autoinducer detection system in mammalian cells. Biol. Proced. Online 6, 268–276 (2004).

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