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A novel method to analyze leukocyte rolling behavior in vivo

Biological Procedures Online volume 6, pages 173–179 (2004)Cite this article

Abstract

Leukocyte endothelial cell interaction is a fundamentally important process in many disease states. Current methods to analyze such interactions include the parallel-plate flow chamber and intravital microscopy. Here, we present an improvement of the traditional intravital microscopy that allows leukocyte-endothelial cell interaction to be studied from the time the leukocyte makes its initial contact with the endothelium until it adheres to or detaches from the endothelium. The leukocyte is tracked throughout the venular tree with the aid of a motorized stage and the rolling and adhesive behavior is measured off-line. Because this method can involve human error, methods to automate the tracking procedure have been developed. This novel tracking method allows for a more detailed examination of leukocyte-endothelial cell interactions.

Abbreviations

ICAM-1:

Intercellular Adhesion Molecule-1

LFA-1:

Lymphocyte Function-Associated Antigen-1

Mac-1:

Macrophage-1

TNF-α:

Tumor Necrosis Factor-α

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Author information

Author notes

  1. Jessica L. Dunne & Klaus Ley

    Present address: Johnson & Johnson Pharmaceutical R & D, L.L.C., 1000 Route 202, 08869, Raritan, NJ

Authors and Affiliations

  1. Cardiovascular Research Center and Department of Biomedical Engineering, University of Virginia, PO Box 800759, 22908, Charlottesville, VA

    Klaus Ley

  2. Department of Electrical and Computer Engineering, University of Virginia, PO Box 400743, 22904, Charlottesville, VA

    Adam P. Goobic & Scott T. Acton

  3. Cardiovascular Research Center and Department of Biomedical Engineering, University of Virginia, PO Box 800759, 22908, Charlottesville, VA

    Jessica L. Dunne

Authors
  1. Jessica L. Dunne
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  2. Adam P. Goobic
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  3. Scott T. Acton
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  4. Klaus Ley
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Corresponding author

Correspondence to Klaus Ley.

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Published: August 27, 2004.

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Dunne, J.L., Goobic, A.P., Acton, S.T. et al. A novel method to analyze leukocyte rolling behavior in vivo . Biol. Proced. Online 6, 173–179 (2004). https://doi.org/10.1251/bpo87

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  • DOI: https://doi.org/10.1251/bpo87

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Biological Procedures Online

ISSN: 1480-9222

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