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Biological Procedures Online

Open Access

A novel method to analyze leukocyte rolling behavior in vivo

  • Jessica L. Dunne1, 2,
  • Adam P. Goobic3,
  • Scott T. Acton3 and
  • Klaus Ley4, 2Email author
Biological Procedures Online6:610173

Received: 20 May 2004

Accepted: 12 August 2004


Leukocyte endothelial cell interaction is a fundamentally important process in many disease states. Current methods to analyze such interactions include the parallel-plate flow chamber and intravital microscopy. Here, we present an improvement of the traditional intravital microscopy that allows leukocyte-endothelial cell interaction to be studied from the time the leukocyte makes its initial contact with the endothelium until it adheres to or detaches from the endothelium. The leukocyte is tracked throughout the venular tree with the aid of a motorized stage and the rolling and adhesive behavior is measured off-line. Because this method can involve human error, methods to automate the tracking procedure have been developed. This novel tracking method allows for a more detailed examination of leukocyte-endothelial cell interactions.

Indexing terms

AnimalsCell AdhesionLeukocytesMice, KnockoutTumor Necrosis FactorVenules