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Biological Procedures Online

Open Access

Flow cytometry based techniques to study testicular acidophilic granulocytes from the protandrous fish gilthead seabream (Sparus aurata L.)

  • Elena Chaves-Pozo1,
  • Victoriano Mulero1,
  • José Meseguer1 and
  • Alfonsa García Ayala1Email author
Biological Procedures Online6:610129

Received: 17 March 2004

Accepted: 11 June 2004


The gilthead seabream is a protandrous seasonal breeding teleost that is an excellent model for studying the testicular regression process which occurs in both seasonal testicular involution and sex reversion. Little is known about the cell types and the molecular mechanisms involved in such processes, mainly because of the lack of appropriate methods for testis dissociation, and testicular cell isolation, culture and functional characterization. We have previously reported that gilthead seabream acidophilic granulocytes infiltrate the testis at post-spawning stage, settle close to the spermatogonia and accumulate intracellular interleukin-1β. In this paper, we report several flow cytometry based assays which allow to establish the role played by gilthead seabream testicular acidophilic granulocytes and permits their quantification.

Indexing terms

TestisFlow cytometryPhagocytosis