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Organotypic cocultures as skin equivalents: A complex and sophisticated in vitro system
Biological Procedures Online volume 6, pages 55–60 (2004)
Abstract
To assess the role of genes required for skin organogenesis, tissue regeneration and homeostasis, we have established in vitro skin equivalents composed of primary cells or cell lines, respectively. In these organotypic cocultures keratinocytes generate a normal epidermis irrespective of the species and tissue origin of fibroblasts. The combination of cells derived from mouse and human tissues facilitates the identification of the origin of compounds involved in epidermal tissue reconstitution and thus the precise analysis of growth regulatory mechanisms.
Abbreviations
- DMEM:
-
Dulbecco’s modified Eagle’s medium
- EGF:
-
epidermal growth factor
- FCS:
-
fetal calf serum
- HDF:
-
human dermal fibroblasts
- MEF:
-
mouse embryonic fibroblasts
- NEK:
-
normal epidermal keratinocytes
- TGF-α:
-
transforming growth factor alpha
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Published: April 12, 2004
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Stark, HJ., Szabowski, A., Fusenig, N.E. et al. Organotypic cocultures as skin equivalents: A complex and sophisticated in vitro system. Biol. Proced. Online 6, 55–60 (2004). https://doi.org/10.1251/bpo72
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DOI: https://doi.org/10.1251/bpo72