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Methods for the detection of d-amino-acid oxidase


Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.


  1. 1.

    Krebs, H.A. 1935. CXCVII. Metabolism of amino-acids. III. Deamination of amino-acids. Biochem. J. 29, 1620–1644.

    PubMed  CAS  Google Scholar 

  2. 2.

    Meister, A. 1965. Biochemistry of the Amino Acids, 2nd ed, vol. 1. Academic Press, New York.

    Google Scholar 

  3. 3.

    Konno, R., Yasumura, Y. 1992. D-Amino-acid oxidase and its physiological function. Int. J. Biochem. 24, 519–524.

    PubMed  Article  CAS  Google Scholar 

  4. 4.

    Watanabe, T., Motomura, Y., Suga, T. 1978. A new colorimetric determination of D-amino acid oxidase and urate oxidase activity. Anal. Biochem. 86, 310–315.

    PubMed  Article  CAS  Google Scholar 

  5. 5.

    Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  6. 6.

    Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.

    PubMed  Article  CAS  Google Scholar 

  7. 7.

    Chomczynski, P., Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.

    PubMed  Article  CAS  Google Scholar 

  8. 8.

    Sambrook, J., Fritsch, E.F., Maniatis, T. 1989. Molecular cloning: a laboratory manual., 2nd ed. Cold Spring Harbor Laboratory Press, New York.

    Google Scholar 

  9. 9.

    Feinberg, A.P., Vogelstein, B. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13.

    PubMed  Article  CAS  Google Scholar 

  10. 10.

    Konno, R., Yasumura, Y. 1981. Activity and substrate specificity of D-amino acid oxidase in kidneys of various animals. Zool. Mag., Tokyo 90, 368–373.

    CAS  Google Scholar 

  11. 11.

    Konno, R., Uchiyama, S., Yasumura, Y. 1982. Intraspecies and interspecies variations in the substrate specificity of D-amino acid oxidase. Comp. Biochem. Physiol. 71B, 735–738.

    CAS  Google Scholar 

  12. 12.

    Konno, R., Yamamoto, K., Niwa, A., Yasumura, Y. 1991. Presence of D-amino-acid oxidase protein in mutant mice lacking D-amino-acid oxidase activity. Int. J. Biochem. 23, 1301–1305.

    PubMed  Article  CAS  Google Scholar 

  13. 13.

    Konno, R., Sasaki, M., Asakura, S., Fukui, K., Enami, J., Niwa, A. 1997. D-Amino-acid oxidase is not present in the mouse liver. Biochim. Biophys. Acta 1335, 173–181.

    PubMed  CAS  Google Scholar 

  14. 14.

    Konno, R. 1998. Rat D-amino-acid oxidase cDNA: rat D-amino-acid oxidase as an intermediate form between mouse and other mammalian D-amino-acid oxidases. Biochim. Biophys. Acta 1935, 165–170.

    Google Scholar 

  15. 15.

    Nakano, S. 1961. Distribution of D-amino acid oxidase in mammalian tissues (in Japanese). Vitamin 22, 306–312.

    CAS  Google Scholar 

  16. 16.

    Katagiri, M., Tojo, H., Horiike, K., Yamano, T. 1991. Immunochemical relationship of D-amino acid oxidase in various tissues and animals. Comp. Biochem. Physiol. 99B, 345–350.

    CAS  Google Scholar 

  17. 17.

    Sasaki, M., Konno, R., Nishio, M., Niwa, A., Yasumura, Y., Enami, J. 1992. A single-base-pair substitution abolishes D-amino-acid oxidase activity in the mouse. Biochim. Biophys. Acta 1139, 315–318.

    PubMed  CAS  Google Scholar 

  18. 18.

    Konno, R., Yasumura, Y. 1983. Mouse mutant deficient in D-amino acid oxidase activity. Genetics 103, 277–285.

    PubMed  CAS  Google Scholar 

  19. 19.

    Konno, R., Yasumura, Y. 1984. Brain and kidney D-amino acid oxidases are coded by a single gene in the mouse. J. Neurochem. 42, 584–586.

    PubMed  Article  CAS  Google Scholar 

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Correspondence to Ryuichi Konno.

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Konno, R. Methods for the detection of d-amino-acid oxidase. Biol Proced Online 1, 27–31 (1998).

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