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T cell integrin overexpression as a model of murine autoimmunity


Integrin adhesion molecules have important adhesion and signaling functions. They also play a central role in the pathogenesis of many autoimmune diseases. Over the past few years we have described a T cell adoptive transfer model to investigate the role of T cell integrin adhesion molecules in the development of autoimmunity. This report summarizes the methods we used in establishing this murine model. By treating murine CD4+ T cells with DNA hypomethylating agents and by transfection we were able to test thein vitro effects of integrin overexpression on T cell autoreactive proliferation, cytotoxicity, adhesion and trafficking. Furthermore, we showed that the ability to inducein vivo autoimmunity may be unique to the integrin lymphocyte function associated antigen-1 (LFA-1).


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Correspondence to Raymond L. Yung.

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Published: October 24, 2003

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Yung, R.L., Ray, D., Mo, R.R. et al. T cell integrin overexpression as a model of murine autoimmunity. Biol. Proced. Online 5, 211–221 (2003).

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Indexing terms

  • Integrins
  • DNA methylation