Open Access

T cell integrin overexpression as a model of murine autoimmunity

Biological Procedures Online5:51211

https://doi.org/10.1251/bpo64

Received: 6 August 2003

Accepted: 10 October 2003

Abstract

Integrin adhesion molecules have important adhesion and signaling functions. They also play a central role in the pathogenesis of many autoimmune diseases. Over the past few years we have described a T cell adoptive transfer model to investigate the role of T cell integrin adhesion molecules in the development of autoimmunity. This report summarizes the methods we used in establishing this murine model. By treating murine CD4+ T cells with DNA hypomethylating agents and by transfection we were able to test thein vitro effects of integrin overexpression on T cell autoreactive proliferation, cytotoxicity, adhesion and trafficking. Furthermore, we showed that the ability to inducein vivo autoimmunity may be unique to the integrin lymphocyte function associated antigen-1 (LFA-1).

Indexing terms

IntegrinsDNA methylation

Notes

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