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Dealing with different methods for Kluyveromyces lactis β-galactosidase purification


Several micro-scale chromatography-based procedures for purification of the β-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.


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Correspondence to M. I. González Siso.

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Becerra, M., Cerdán, E. & González Siso, M.I. Dealing with different methods for Kluyveromyces lactis β-galactosidase purification. Biol Proced Online 1, 48–58 (1998).

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  • Lactase
  • Cheese Whey
  • Purification Factor
  • Biological Procedure
  • Crude Protein Extract