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Biological Procedures Online

Open Access

Dealing with different methods for Kluyveromyces lactis β-galactosidase purification

Biological Procedures Online1:110048


Several micro-scale chromatography-based procedures for purification of the β-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.


LactaseCheese WheyPurification FactorBiological ProcedureCrude Protein Extract