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Dealing with different methods for Kluyveromyces lactis β-galactosidase purification
Biological Procedures Onlinevolume 1, pages48–58 (1998)
Several micro-scale chromatography-based procedures for purification of the β-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.
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