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Dealing with different methods for Kluyveromyces lactis β-galactosidase purification

Biological Procedures Online volume 1, pages 48–58 (1998)Cite this article

Abstract

Several micro-scale chromatography-based procedures for purification of the β-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.

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Authors and Affiliations

  1. Departamento de BioloxÍa Celular e Molecular, Facultade de Ciencias, Campus da Zapateira s/n, 15071, A Coruña, Spain

    M. Becerra, E. Cerdán & M. I. González Siso

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  1. M. Becerra
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  2. E. Cerdán
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  3. M. I. González Siso
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Correspondence to M. I. González Siso.

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Becerra, M., Cerdán, E. & González Siso, M.I. Dealing with different methods for Kluyveromyces lactis β-galactosidase purification. Biol Proced Online 1, 48–58 (1998). https://doi.org/10.1251/bpo4

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  • DOI: https://doi.org/10.1251/bpo4

Keywords

  • Lactase
  • Cheese Whey
  • Purification Factor
  • Biological Procedure
  • Crude Protein Extract

Biological Procedures Online

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