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Biological Procedures Online

Open Access

Methods for direct determination of mitomycin C in aqueous solutions and in urine

  • Dolores Marín1Email author,
  • Pedro Pérez1,
  • Carmen Teijeiro1 and
  • Emil Paleček2
Biological Procedures Online1:120100


Stripping voltammetry (SV) is used to quantitatively determine concentrations of the anti-neoplastic drug mitomycin C (MMC) alone and in mixtures with 5-fluorouracil and cisplatin, both of which are used in combined chemotherapy withMMC. If the accumulation is performed at the potentials of MMC reduction (−0.35Vvs. SCE), reduced MMC is strongly adsorbed at the electrode. It is possible to prepare a MMCmodified electrode, which, after a washing step, is transferred to the background electrolyte to determine MMC by voltammetry. This procedure, which is termed transfer stripping voltammetry (TSV), helps to eliminate interferences and can be applied for a direct determination ofMMCalone or in mixtures with other drugs in urine.


Urine SampleMitomycinSaturated Calomel ElectrodeBackground ElectrolyteAccumulation Potential