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Methods for direct determination of mitomycin C in aqueous solutions and in urine

Biological Procedures Online volume 1, pages 100–106 (1998)Cite this article

Abstract

Stripping voltammetry (SV) is used to quantitatively determine concentrations of the anti-neoplastic drug mitomycin C (MMC) alone and in mixtures with 5-fluorouracil and cisplatin, both of which are used in combined chemotherapy withMMC. If the accumulation is performed at the potentials of MMC reduction (−0.35Vvs. SCE), reduced MMC is strongly adsorbed at the electrode. It is possible to prepare a MMCmodified electrode, which, after a washing step, is transferred to the background electrolyte to determine MMC by voltammetry. This procedure, which is termed transfer stripping voltammetry (TSV), helps to eliminate interferences and can be applied for a direct determination ofMMCalone or in mixtures with other drugs in urine.

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Authors and Affiliations

  1. Departamento de QuÍmica FÍsica, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain

    Dolores Marín, Pedro Pérez & Carmen Teijeiro

  2. Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 61265, Brno, Czech Republic

    Emil Paleček

Authors
  1. Dolores Marín
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  2. Pedro Pérez
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  3. Carmen Teijeiro
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  4. Emil Paleček
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Correspondence to Dolores Marín.

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Marín, D., Pérez, P., Teijeiro, C. et al. Methods for direct determination of mitomycin C in aqueous solutions and in urine. Biol. Proc. Online 1, 100–106 (1998). https://doi.org/10.1251/bpo12

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  • DOI: https://doi.org/10.1251/bpo12

Keywords

  • Urine Sample
  • Mitomycin
  • Saturated Calomel Electrode
  • Background Electrolyte
  • Accumulation Potential

Biological Procedures Online

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