Open Access

Assay for high glucose-mediated islet cell sensitization to apoptosis induced by streptozotocin and cytokines

Biological Procedures Online7:710162

https://doi.org/10.1251/bpo113

Received: 3 August 2005

Accepted: 11 October 2005

Abstract

Pancreatic β-cell apoptosis is known to participate in the β-cell destruction process that occurs in diabetes. It has been described that high glucose level induces a hyperfunctional status which could provoke apoptosis. This phenomenon is known as glucotoxicity and has been proposed that it can play a role in type 1 diabetes mellitus pathogenesis. In this study we develop an experimental design to sensitize pancreatic islet cells by high glucose to streptozotocin (STZ) and proinflammatory cytokines [interleukin (IL)-1β, tumor necrosis factor (TNF)-α and interferon (IFN)-γ]-induced apoptosis. This method is appropriate for subsequent quantification of apoptotic islet cells stained with Tdt-mediated dUTP Nick-End Labeling (TUNEL) and protein expression assays by Western Blotting (WB).

Indexing terms

Apoptosis Diabetes Mellitus Streptozotocin Cytokines

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