Skip to main content
  • Published:

Yeast exoglycoproteins produced under NaCl-stress conditions as efficient cryoprotective agents

Abstract

Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. Glycoproteins secreted by the strain Dipodascus australiensis into growth medium containing NaCl (8% w/v) were found to be the most effective cryoadditives. It was possible to use these glycoproteins alone (without DMSO as penetrating agent) for the cryoprotection of the studied yeasts.

References

  1. Nakagawa S., Ouchi K. 1994. Improvement of freeze tolerance of commercial baker’s yeasts in dough by heat treatment before freezing. Bioscience, Biotechnology and Biochemistry 11, 2077–2079.

    Article  Google Scholar 

  2. Berendsen H. J. C. 1975. Water a comprehensive treatise. F. Franks, New York: Plenum, 293–330, 5

    Google Scholar 

  3. Tan C. S., Van Ingen C.S., Talsma M. et al. 1995. Freeze-drying of fungi: influence of composition and glass transition temperature of the protectant. Cryobiology 32, 60–67.

    Article  Google Scholar 

  4. Breierova E., Kockova-Kratochvilova A. 1992. Cryoprotective effects of yeast extracellular polysaccharides and glycoproteins. Cryobiology 29, 385–390.

    Article  PubMed  CAS  Google Scholar 

  5. Korber Ch., Scheiwe M. W., Boutron P. 1982 Can the stability of the completely amporphous state explain the cryoprotection of hydroxyethyl starch? Cryo-Letters 3, 83–90.

    Google Scholar 

  6. Franks F. 1982. Apparent osmotic activities of water soluble polymers used a cryoprotectant. Cryo-Letters 3, 115–120.

    CAS  Google Scholar 

  7. Tanner W. Lehle L. 1987. Protein glycosylation in yeast. Biochimica et Biophysica Acta 906, 81–89.

    PubMed  CAS  Google Scholar 

  8. Breierova E. 1994. Cryoprotection of psychrophylic yeast species by the use of additives with cryoprotective media. Cryo-Letters 15, 191–197.

    Google Scholar 

  9. Tilser J., Breierova E., Tichy M., et al. 1996. Effect of supplementation of University of Wisconsin solution with glycoproteins from psychrophilic strains of yeast on hypothermic liver storage of rats. Cryobiology 33, 347–353.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to E. Breierova.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Breierova, E. Yeast exoglycoproteins produced under NaCl-stress conditions as efficient cryoprotective agents. Biol Proced Online 1, 10–16 (1998). https://doi.org/10.1251/bpo1

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1251/bpo1

Keywords