Yeast exoglycoproteins produced under NaCl-stress conditions as efficient cryoprotective agents
Biological Procedures Online volume 1, pages 10–16 (1998)
Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. Glycoproteins secreted by the strain Dipodascus australiensis into growth medium containing NaCl (8% w/v) were found to be the most effective cryoadditives. It was possible to use these glycoproteins alone (without DMSO as penetrating agent) for the cryoprotection of the studied yeasts.
Nakagawa S., Ouchi K. 1994. Improvement of freeze tolerance of commercial baker’s yeasts in dough by heat treatment before freezing. Bioscience, Biotechnology and Biochemistry 11, 2077–2079.
Berendsen H. J. C. 1975. Water a comprehensive treatise. F. Franks, New York: Plenum, 293–330, 5
Tan C. S., Van Ingen C.S., Talsma M. et al. 1995. Freeze-drying of fungi: influence of composition and glass transition temperature of the protectant. Cryobiology 32, 60–67.
Breierova E., Kockova-Kratochvilova A. 1992. Cryoprotective effects of yeast extracellular polysaccharides and glycoproteins. Cryobiology 29, 385–390.
Korber Ch., Scheiwe M. W., Boutron P. 1982 Can the stability of the completely amporphous state explain the cryoprotection of hydroxyethyl starch? Cryo-Letters 3, 83–90.
Franks F. 1982. Apparent osmotic activities of water soluble polymers used a cryoprotectant. Cryo-Letters 3, 115–120.
Tanner W. Lehle L. 1987. Protein glycosylation in yeast. Biochimica et Biophysica Acta 906, 81–89.
Breierova E. 1994. Cryoprotection of psychrophylic yeast species by the use of additives with cryoprotective media. Cryo-Letters 15, 191–197.
Tilser J., Breierova E., Tichy M., et al. 1996. Effect of supplementation of University of Wisconsin solution with glycoproteins from psychrophilic strains of yeast on hypothermic liver storage of rats. Cryobiology 33, 347–353.
About this article
Cite this article
Breierova, E. Yeast exoglycoproteins produced under NaCl-stress conditions as efficient cryoprotective agents. Biol Proced Online 1, 10–16 (1998). https://doi.org/10.1251/bpo1