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Biological Procedures Online

Open Access

Yeast exoglycoproteins produced under NaCl-stress conditions as efficient cryoprotective agents

Biological Procedures Online1:10


Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. Glycoproteins secreted by the strain Dipodascus australiensis into growth medium containing NaCl (8% w/v) were found to be the most effective cryoadditives. It was possible to use these glycoproteins alone (without DMSO as penetrating agent) for the cryoprotection of the studied yeasts.


Yeast SpeciesBiological ProcedureOsmotic EquilibriumCryoprotective AgentBiological Procedure Online