Fig. 6
From: Efficient and scalable gene delivery method with easily generated cationic carbon dots
A Transfection efficiency measured as the % of GFP-positive cells analyzed by flow cytometry of HEK293T cells plated in 100 mm diameter dishes and transfected with CCDs generated by incubation of CDs with PEI at a 20:3 mass ratio and mixed with a fixed amount of DNA to obtain the optimized 20:3:1 mass ratio. The standard transfection using PEI at a 3:1 mass ratio with DNA was used as a control (orange) (n = 11, mean values ± SD). Student’s t-test was applied for statistical significance: *** P < 0.001) B Viral transduction efficiency measured as the % of GFP-positive cells analyzed by flow cytometry of HEK293T cells after incubation with viral-containing supernatant secreted by HEK293T cells after three vector cotransfection using either CCD or PEI conditions (n = 9, mean values ± SD. Student’s t-test was applied for statistical significance: ***P < 0.001) C Representative fluorescence microscopy images of viral transduced HEK293T cells as in (B) (scale bar = 100 µm). D Viral transduction efficiency measured as the % of GFP-positive cells analyzed by flow cytometry of MenSC cells after incubation with viral-containing supernatant secreted by HEK293T cells after three vector cotransfection using either CCD or PEI conditions (n = 3, mean values ± SD. Student’s t-test was applied for statistical significance: ***P < 0.001) E Representative fluorescence microscopy images of viral transduced MenSC cells as in (D) (scale bar = 100 µm)