Fig. 3

A Schematic of CCD preparation and cell transfection B Transfection efficiency measured as the % of GFP-positive cells analyzed by flow cytometry of HEK293T cells plated in p6 wells and transfected with CCDs generated by incubation of CDs with PEI at different mass ratios and mixed with a fixed amount of DNA. PEI at a 3:1 mass ratio with DNA was used as a control (orange) (n = 7, mean values ± SD. Student’s t-test was applied for statistical significance: ***P < 0.001). C Representative fluorescence microscopy images of transfected HEK293T cells with CCDs (20:3 mass ratio condition) or PEI (scale bar = 100 µm). D Transfection efficiency measured as the % of GFP-positive cells analyzed by flow cytometry of MenSC cells plated in p6 wells and transfected with CCDs (20:3 mass ratio condition). E Representative fluorescence microscopy images of MenSCs transfected cells with CCDs or PEI (scale bar = 100 µm)