Fig. 7

D-allose inhibits TLR4/PI3K/AKT signaling to attenuate neuroinflammation and apoptosis by inhibiting Gal-3 after MCAO/R injury. A RT-PCR analysis of the mRNA expression of Gal-3 in mice after transfection with AAV.  B Western blotting was performed to detect the protein expression of Gal-3 in mice after AAV transfection. C Immunofluorescence analysis of mice transfected with AAV. D The transfected mice were subjected to MCAO injury and administered D-allose treatment, and their neurological function was scored. E TTC staining was used to observe the changes in infarct volume. F Brain edema was observed in each group of mice after injury. G Western blotting was performed to detect the expression of Gal-3-TLR4 signaling pathway and apoptosis proteins in each group of mice.  (H) TUNEL staining to observe neuronal apoptosis in mouse brain tissue. I, J, K ELISA kits were used to detect the secretion of TNF-α, IL-1β and IL-6 in brain tissue.  ^nsP>0.05, **P<0.01 compared with the Sham group; ^##P<0.01 compared with the MCAO group; ^nsP>0.05, ^&P<0.05, ^&&P<0.01 compared with the MCAO + D-allose group. Data were analyzed using one-way ANOVA, and Dunnett’s test was used for post hoc analysis. The data were from three independent experiments