Fig. 6

D-allose regulates the TLR4/ PI3K/AKT pathway via Gal-3 to protect HT-22 cells from OGD/R-induced injury. A Detection of HT-22 cell viability after transfection with LV and D-allose treatment, as determined by CCK-8 assays. B Measurement of LDH levels in HT-22 cells after transfection with the lentivirus and D-allose treatment. C, D, E Analysis of relevant inflammatory factors in each group of HT-22 cells by ELISA. F Detection of apoptotic proteins such as Bax and related proteins in the Gal-3-TLR4 signaling pathway by Western blot analysis. G TUNEL staining to observe apoptotic cells in each group. **P<0.01 compared with the Con group; ^##P<0.01 compared with the OGD/R group; ^nsP>0.05, ^&&P<0.01 compared with the OGD/R + D-allose group; ^$P<0.05, ^$$P<0.01 compared with the OGD/R + sh-NC group. The data were analyzed using one-way ANOVA and Dunnett’s test was used for post hoc analysis. The data were from three independent experiments