Fig. 5

Gal-3 activates the TLR4/ PI3K/AKT signaling axis in vitro. A Western blot analysis of the expression of related proteins in the Gal-3-TLR4-PI3K-AKT pathway after the administration of D-allose. B Expression of related proteins in the Gal-3-TLR4-PI3K-AKT pathway after lentiviral transfection, as detected by Western blotting. C Western blot analysis of the expression of signaling pathways and apoptosis-related proteins after transfection with the Gal-3 lentivirus and the administration of agonists and inhibitors of TLR4.  D Detection of HT-22 cell viability by CCK-8 assays after transfection with the Gal-3 lentivirus and the administration of agonists and inhibitors of TLR4. E Measurement of LDH levels in HT-22 cells.  F TUNEL staining was used to observe apoptosis in each group of HT-22 cells.  G-I ELISA kits were used to detect the levels of secreted inflammatory factors in the groups of cells. **P<0.01 compared with the Con group; ^nsP>0.05, ^##P<0.01 compared with the OGD/R group; ^&&P<0.01 compared with the OGD/R + sh-Gal-3 group. Data were analyzed using one-way ANOVA and Dunnett’s test was used for post hoc analysis. Data were from three independently conducted experiments