Fig. 4

Gal-3 directly binds with TLR4 in vitro.  A Immunofluorescence analysis of the colocalization of Gal-3 and TLR4 in HT-22 cells. B Immunofluorescence analysis of the colocalization of Gal-3, neurons, and TLR4 in brain tissue. C Analysis of protein structure using 3D structure prediction techniques. A mock structural map of the binding of Gal-3 (cyan) to TLR4 (green) was obtained, as well as the specific binding sites.  D Immunoblot showing the presence of Gal-3 and TLR4 in an immune complex formed after the pull-down of TLR4 and Gal-3 in HT-22 cells in the con group and OGD group