Fig. 3

Increased expression of Gal-3 in ischemic/reperfusion(I/R) injury in vivo and in vitro. A Changes in Gal-3 mRNA levels in brain tissue 24 and 48 h after MCAO, as measured by RT-PCR. B Protein expression of Gal-3 protein content in brain tissue 24 and 48 h after MCAO, as measured by Western blotting. C Observation of Gal-3 expression in brain tissue 24 and 48 h after MCAO by immunofluorescence analysis. D Detection of Gal-3 mRNA expression in HT-22 cells after different lengths of OGD/R injury using RT-PCR. E Examination of Gal-3 expression in HT-22 cells after different durations of OGD/R injury by using Western blotting. F Observation of Gal-3 expression in HT-22 cells after different durations of OGD/R injury, as measured by immunofluorescence analysis. G IF was used to observe lentiviral transfection. H Detection of Gal-3 mRNA expression after lentiviral transfection. I Detection of Gal-3 protein expression after transfection with the lentivirus. J CCK-8 assay to detect cell viability after transfection with the lentivirus. K Detection of LDH levels after lentiviral transfection. L Western blot analysis of caspase 3, Bax, Bcl-2, and caspase 1 protein expression after LV transfection. M TUNEL staining was used to observe HT-22 cell apoptosis after transfection with different lentiviruses. N-P The expression levels of inflammatory factors in HT-22 cells transfected with the lentivirus and then damaged by OGD/R were measured using ELISA kits. ** P<0.01 compared with the Con group; ^nsP>0.05, ^##P<0.01 compared with the OGD/R group. The data were analyzed using one-way ANOVA, and Dunnett’s test was used for post hoc analysis. The data were from three independent experiments