Fig. 6
MSI2 deficiency suppresses CRC malignant features, including tumor proliferation and metastasis, by inhibiting MAPK/HSPB1 axis phosphorylation in vivo. A In vivo models of xenograft tumors from HT29 and LOVO stable cells were established in M-NSG mice, n = 6. B Quantitative analysis of xenografted tumor volume change from HT29 and LOVO stable cells, n = 6. C, Statistical analysis of xenografted tumor weight from HT29 and LOVO stable cells, n = 6. D Procedure for constructing colitis-associated colon cancer in vivo models of MSI2+/+ and MSI2−/− transgenic mice. E Representative image and statistical analysis of colitis-associated colon cancer tumor number in MSI2+/+ and MSI2−/− transgenic mice, n = 5. F Representative H&E, IHC images and statistical analysis of the IHC score (IOD per area) of MSI2, HSPB1, p-HSPB1(Ser78) and Ki67 in MSI2+/+ and MSI2−/− transgenic CAC mice model. Scale bars, H&E, 200 μm; IHC, 100 μm. G Representative H&E and IHC images of xenograft tumors from SW620, LOVO and HT29 stable cells, including MSI2, HSPB1, p-HSPB1(Ser78), p-P38, ACSL4, Ki67 and PCNA. Scale bars, H&E, 200 μm; IHC, 100 μm. H Statistical analysis of the IHC score (IOD per area) of MSI2, HSPB1, p-HSPB1(Ser78), p-P38, ACSL4, Ki67 and PCNA from SW620, LOVO and HT29 stable cells xenograft tumors. I-J Representative images and statistical analysis of liver metastasis nodules from SW620 and LOVO stable cells in M-NSG mice, n = 5. K Representative images of H&E staining of liver metastasis from SW620 and LOVO stable cells. The yellow star indicates the tumor area. Scale bars, 200 μm. L-M Representative images and statistical analysis of lung metastasis nodules from SW620 and LOVO stable cells in M-NSG mice, n = 5. N Representative images of H&E staining of lung metastasis from SW620 and LOVO stable cells. The dark star indicates the tumor area. Scale bars, 400 μm. These results are presented as the mean ± SD values; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; B, C, E, F, H, J, M unpaired 2-tailed Student’s t test