Fig. 4

MSI2 dysregulates HSP family gene expression and multiple signal transduction processes. A Principal component analysis (PCA) plot of stable SW620-SC and SW620-SH cells in the proteomics analysis. B Pearson correlation analysis between stable SW620-SC and SW620-SH cells in the proteomics analysis. C The distribution, localization and proportion of significantly genes corresponding to differentially expressed proteins in SW620 cell proteomics. D In total, 460 upregulated protein genes and 391 downregulated protein genes were identified. E Heatmap of all the genes corresponding to differentially expressed proteins identified by proteomics. F Heatmap of differential HSP family protein genes identified in proteomics. G Circular heatmap demonstrating the expression of the most differentially expressed HSP family protein gene in proteomics. H The downregulated Gene set enrichment analysis (GSEA) of KEGG pathway was enriched in the MAPK signaling pathway. I, The positive correlation between MSI2 expression and HSPB1 expression was analyzed by Spearman correlation analysis and normalized by GAPDH in the GEPIA COAD and READ database (p = 6.2e-08, R = 0.28), n = 367. J GSEA analysis of differential proteins revealed the most significantly downregulated KEGG pathways, such as oxidative phosphorylation, Huntington’s disease, Parkinson’s disease, Alzheimer’s disease and ferroptosis suppressor genes set, while the ferroptosis driver genes set was upregulated, and ferroptosis-related genes set were obtained from FerrDb databases. K The up-regulated GO molecular functions (MFs) were enriched in metal ion binding and ion channel binding. L Associations between MSI2 expression and aberrant signaling pathway score from TCGA CRC datasets (n = 620), such as the positive correlation with tumor proliferation signature (R = 0.19), the negative correlations with genes upregulated by ROS (R=-0.36) and glutathione metabolism (R=-0.27)