Fig. 2

MSI2 deficiency inhibits the proliferation, migration and invasion of CRC cells in vitro. A Western blotting for MSI2 expression in SW620, LOVO, and HT29 stable cells. B MSI2 mRNA expression was determined by qRT‒PCR in SW620, LOVO, and HT29 stable cells. C, The cell viability and proliferation were monitored by CCK-8 at 0–96 h in SW620, LOVO, and HT29 stable cells. D-E Representative images and statistical analysis of the colony formation assay of stable cell lines showed that MSI2 deficiency significantly reduced colony formation. F-G Representative images and statistical analysis of Transwell migration and invasion of SW620, LOVO, and HT29 stable cell lines showed that MSI2 deficiency significantly inhibited migration and invasion. H FACS analysis of the mitochondrial membrane potential assay with JC-1 showed that MSI2 deficiency significantly increased JC-1 monomer (PE⁻ fluorescence) percentages. I Statistical FACS analysis percentages of JC-1 monomers (PE⁻ fluorescence) and JC-1 aggregates (PE⁺ fluorescence) in SW620 and LOVO stable cells. J Representative IFC images of the mitochondrial membrane potential assay with JC-1 in SW620 and LOVO stable cells. Blue: DAPI, red: JC-1 (PE), green: JC-1 (FITC); Scale bars, 200 μm. K Statistical IFC analysis of JC-1 aggregates and JC-1 monomers in SW620 and LOVO stable cells. These results are presented as the mean ± SD values; *p < 0.05, ***p < 0.001, ****p < 0.0001; B, C, E, G, I, K unpaired 2-tailed Student’s t test