Fig. 7
Effect of R1-MVs on the Nrf2/ARE pathway in H2O2-treated HaCaT cells. HaCaT cells were pre-processed with R1-MVs at different concentrations (10 and 30 μg/mL) for 12 h prior to exposure to H2O2 (0.3 mM) for 12 h. A The expression of Nrf2 and Keap1 in the cytoplasm and nucleus and the total levels of Nrf2 measured using western blotting analysis. B The relative Nrf2 and Keap1 band intensity of each protein evaluated using ImageJ software and expressed as a percentage. *p < 0.05 or ***p < 0.001 vs. H2O2-treated group. The data show the mean ± SD (n = 4 samples) of three representative experiments