Fig. 6
Effects of R1-MVs on the activation of the MAPK pathway in H2O2-treated HaCaT cells. HaCaT cells were pre-processed with R1-MVs at different concentrations (10 and 30 μg/mL) for 12 h prior to exposure to H2O2 (0.3 mM) for 12 h. A The expression of phosphor-p38 (p-p38), p38, p-ERK, ERK, p-JNK, JNK, and β-actin was measured using western blotting analysis. B The relative MAPK band intensity of each protein evaluated using ImageJ is expressed as a percentage. ###p < 0.001 vs. control group; *p < 0.05, **p < 0.01, or ***p < 0.001 vs. H2O2-treated group. The data show the mean ± SD (n = 4 samples) of three representative experiments