Fig. 5
Effect of R1-MVs on MMP and expression of apoptotic proteins in H2O2-treated HaCaT cells. HaCaT cells were pre-processed with R1-MVs at different concentrations (10 and 30 μg/mL) for 12 h prior to exposure to H2O2 (0.3 mM) for 12 h. A The change in MMP was detected by JC-1 using a 20 × laser scanning confocal microscope. Scale bar: 50 nm. B Percentage of fluorescence intensity specific value of the red/green quantified by ImageJ software. Significance: ###p < 0.001 vs. control; *p < 0.05 and **p < 0.01 vs. H2O2-treated group. C Expression of proteins related to apoptosis signals using specific antibodies against Bax, Bcl-2, Cytochrome C (cytosol), Cleaved Caspase 3, Cleaved Caspase 8, Cleaved Caspase 9, Cleaved poly (ADP-ribose) polymerase (PARP), and β-actin. D The relative band intensity of each protein evaluated using ImageJ software and expressed as a percentage. ###p < 0.001 vs. control group; *p < 0.05, **p < 0.01, or ***p < 0.001 vs. H2O2-treated group. The values are mean ± SD (n = 4 samples) of three representative experiments