Fig. 5

TLR9 can be modulated by miR-342-5p. Notes: The binding site of miR-342-5p and TLR9 was predicted by TargetScan, and the 3’-UTR region of miR-342-5p and TLR9 was constructed (A). Dual-luciferase reporter assay confirmed the interaction between TLR9 and miR-342-5p (B). Subsequently, the HK-2 cells were transfected with miR-342-5p mimic, miR-67 mimic, miR-342-5p inhibitor, or miR-67 inhibitor. qRT-PCR (C) and western blot (D, E) were used to detect the mRNA and protein expressions of TLR9. After HK-2 cells were exposed to AMSC-Exo-342 or transfected with pcDNA3.1-TLR9 prior to treatment with AMSC-Exo-342, the mRNA and protein expressions of TLR9 in HK-2 cells were assessed by qRT-PCR (F) and western blot (G, H); N = 3. ^** P < 0.01, compared to the mimic NC + wt-TLR9 group, miR-67 mimic group, miR-67 inhibitor group, AMSC-Exo-67 group, or AMSC-Exo-342 + pcDNA3.1 group; UTR, untranslated region; AMSCs, adipose-derived mesenchymal stem cells; Exo, exosome. Data were analyzed using one-way analysis of variance and Tukey test was used for post hoc analysis. Data were generated from three independent experiments