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Fig. 1 | Biological Procedures Online

Fig. 1

From: Blocking Abundant RNA Transcripts by High-Affinity Oligonucleotides during Transcriptome Library Preparation

Fig. 1

Overview of targeted transcripts and investigated library preparation methods. A Visual representation of the evaluated RNA sequencing library preparation methods with the associated blocked transcripts. B-F Coverage plots of standard library prep RNA-sequencing data for the targeted region. The height of the bars represents the relative number of reads mapping to that position in the transcript. The LNA oligonucleotide and its binding location are shown in red/green under the coverage plot. The targeted transcripts and their chromosomal location are indicated at the bottom of each plot. G Schematic overview of blocking during RT and PCR amplification

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