Fig. 8

Ultrasound/microbubble-mediated miR-424-5p delivery blocks PE development in mice. Note: Mice were subcutaneously injected with L-NAME and treated with ultrasound/microbubbles loaded with miR-424-5p overexpression plasmids. The systolic pressure of mice was evaluated on GD 6.5 (A) and GD 18.5 (B); the proteinuria of mice was detected on GD 18.5 (C); the histopathological changes in mouse placental tissues were evaluated by hematoxylin and eosin staining (D); qRT-PCR was used to detect miR-424-5p expression in mouse placental tissues (E); qRT-PCR and western blot were used to measure AOC1 mRNA (F) and protein (G) levels in mouse placentae. N = 6, * P < 0.05, **P < 0.01, ***P < 0.001. PE, preeclampsia; GD, gestational day