Fig. 3

Light microscopy images of differentiation SCAPs cells to cementoblast, with Alizarin red staining assay. a Control cells on day 7 of cell culture in DMEM solution with 10% FBS and 1% penicillin/streptomycin.; b Differentiated cells on day 7 of cell culture in DMEM with 10% FBS, penicillin/streptomycin 1%, 10 mM of ß-glycerophosphate, and 50 g/mL of ascorbic acid 2-phosphate.; c Control cells on day 14 of cell culture in DMEM solution with 10% FBS and 1% penicillin/streptomycin.; d Differentiated cells on day 14 of cell culture in DMEM with 10% FBS, penicillin/streptomycin 1%, 10 mM of ß-glycerophosphate, and 50 g/mL of ascorbic acid 2-phosphate.; e Control group on day 21 of cell culture in DMEM solution with 10% FBS and 1% penicillin/streptomycin.; f Differentiated cells on day 21 in DMEM with 10% FBS, penicillin/streptomycin 1%, 10 mM of ß-glycerophosphate, and 50 g/mL of ascorbic acid 2-phosphate.; g Stained control cells on day 21, and (h) Stained differentiation SCAPs cells on day of 21