Fig. 12

In vitro validation of PRKCD on HCC cells proliferation, invasion and migration. A-B Measurement of both mRNA and protein expression levels of PRKCD in HepG2 and MHCC97H cells transfected with two PRKCD siRNA sequences, si-NC was utilized as the negative control. C Effects of si-PRKCD and platelet stimulation on proliferation abilities of HepG2 and MHCC97H cells measured by the CCK-8 assay. D Effects of si-PRKCD and platelet stimulation on proliferation abilities of HepG2 and MHCC97H cells measured by the colony formation test. E The transwell assay was performed to assess the impacts of si-PRKCD and platelet stimulation on HCC cells migration (upper) and invasion (below) capacities. Scale bar: 200 μm (200 ×). F The wound healing test displayed the migration ability of HCC cells undergone different treatments. Scale bar: 100 μm (40 ×). The results were presented with representative images from three times independent replicate experiments, and all data were shown as Means ± SD. (**p < 0.01; ***p < 0.001)