Fig. 5

Ang-1–7 sensitizes the response to chemotherapy and immunotherapy. A Proliferative potential of MDA-MB-231 cells was checked using CCK-8 assay after ACE2 overexpression. B The transcriptional level of PD-L1 was checked using qPCR after ACE2 overexpression. C The protein level of PD-L1 was checked using Western blotting after ACE2 overexpression. D Schematic protocol of the combinated therapy in immunocompetent BLAB/c mice. E Tumor volume was monitored every three days. Significance was calculated with One-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05, ***P < 0.001. F The tumor weight of isolated tumors from these six groups, mice were sacrificed on day 25 after treatment. Significance was calculated with One-way ANOVA with Tukey’s multiple comparisons test. ***P < 0.001, ****P < 0.0001. G Relative infiltration level of CD3⁺ and CD8⁺ T cells in control and Ang-1–7 treated tumors. Magnification, 200 × . H Schematic diagram of time point of plasma sample collection. I Comparison of plasma Ang-1–7 levels pre-and post-chemotherapy. Significance was calculated with Student’s t-test. ns-P > 0.05. J Correlation between the response to neoadjuvant chemotherapy and plasma Ang-1–7 levels pre-and post-chemotherapy. Significance was calculated with Pearson correlation analysis