Fig. 4

Circ_0003570 suppresses HCC proliferation, migration and invasion via the miR-182-5p/STARD13 axis. A The results of MTT assay showed that miR-182-5p mimics restored the inhibition of LV-circ_0003570 on cell proliferation over time. HCC cells grew faster in LV-circ_0003570 and miR-182-5p mimics co‐transfection group than LV-circ_0003570 group. *P < 0.05. B Colony formation assay showed that the proliferative capability of HCC cells was increased by miR-182-5p mimics compared with LV-circ_0003570 group. *P < 0.05. C, D The migration and invasion capacity restrained by upregulation of circ_0003570 could be reversed by miR‐182‐5p mimics. Cells migrated and invaded were increased in LV-circ_0003570 and miR-182-5p mimics co‐transfection group. *P < 0.05; **P < 0.01. E, F In wound healing assay, miR‐182‐5p mimics reversed the inhibitory role of LV-circ_0003570 on migration in HepG2 and MHCC-97L cells. *P < 0.05. G In protein level, the upregulation of STARD13 regulated by LV-circ_0003570 could be rescued by miR‐182‐5p mimics. One-way ANOVA was performed to compare the difference between two groups