Fig. 4

Upregulation of KIF20A promotes tumor progression in BCa. A A Venn diagram of the six commonly up-regulated DEGs (KIF20A, CDK1, CAPG, NOP10, PKM, IP6K2) in KDELR2 high expression group in the three independent GEO datasets (GSE3167, GSE52519 and GSE65635). B A Venn diagram of the commonly down-regulated DEGs (CRP) in KDELR2 low expression group. C-D Expression profiles of KIF20A, CAPG, and CDK1 mRNA in the siKDELR2 or KDELR2-overexpressing and control group UMUC3 and T24 cell lines (n = 3). E The correlation of KDELR2 mRNA levels with KIF20A in BCa (n = 408). F Detection of KDELR2 and KIF20A protein expressions by western blot analysis in the siKDELR2 or KDELR2-overexpressing group (n = 3). G Verification of KIF20A expression in clinical tissues by qRT-PCR (n = 24). H Detection of KIF20A expression by western blot analysis in cell lines (n = 3). I Proliferation analysis of UMUC3 or T24 cells in the control, siKIF20A-1, and siKIF20A-3 groups (n = 4). J Migration and invasion (200x) of BCa cells in the control, siKIF20A-1, and siKIF20A-3 groups (n = 3). p < 0.05, *; p < 0.01, **; p < 0.001, ***; p < 0.0001, ****