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Table 2 Individual parameter values measured in the selected single metastatic ALN (mALN) for each of the BC patient presented in Table 1 and described in Methods and Fig. 4

From: Establishment and Fractionation of Metastatic Axillary Lymph Node Cell Suspension for Determination of Protein Expression Levels of Nuclear cFOS and Cytosolic TGFβ1 from Breast Cancer Patients

Parameter

Patients’ number

1

2

3

4

5

6

Diameter of tumor depositsa (mm)

80.0b

9.5

14.5

13.5

1.8

n.a.d

% of malignant cells

80%

100%

100%

100%

30%

n.a.

Presence of micrometastasisc

none

none

none

none

positive

n.a.

Initial tissue weight (mg)

790

720

190

660

630

530

Total viable cell number (×  10− 6)

18.6

3.6

5.2

9.0

3.8

3.9

Nuclear protein yield (mg)

3.2

0.6

1.0

1.7

1.1

0.8

Cytosolic protein yield (mg)

6.5

1.2

1.7

2.5

0.9

1.2

cFOS level (%)

15.50

8.75

13.20

0.00

0.00

0.00

TGFβ1 level (ng/mg protein)

3.60

1.20

0.90

1.02

0.67

1.08

  1. aThe size of tumor deposits was assessed from the H&E slices of formalin-fixed, paraffin-embedded ALN tissue
  2. b“mALN1” essentially exhibited the conglomerate size: 80 × 50 × 40 mm, with malignant infiltration of surrounding ALN tissue
  3. cobserved morphologically
  4. dnot available due to much too small ALN size and the entire “mALN 6” was used for cFOS and TGFβ1 analysis