Fig. 2

Restriction maps of transformation vectors and the results of Agrobacterium infiltration in African violet petals. a Binary vectors. The cloning vector pBI121 and pCAMBIA1304 containing the CaMV 35 S promoter driving the 4’CGT and AS1 genes respectively. b Agroinfiltration steps in African violet petals (1): The injection of Agrobacterium infiltration solution in the base of the petal by syringe without a needle, blue arrows without injection, a green arrow without gene construct and orange arrow with gene construct 4’CGT+AS1; (2) a color change from white to yellow in petal influenced by gene construct 4’CGT+AS1; (3, 4): Microscopes in the petal color injected samples with a gene construct and without construct; Light microscopes in the sample with a gene construct (5) and without a gene construct (6); (7): Shown are petal color on the adaxial side of sample injection with a gene construct; (8): Cross-sections arrow show that fluorescence is restricted to the pigmented adaxial epidermis of gene construct (Scalebar:100). c The results of extraction DNA and PCR detection of agroinfiltration petals; M: GeneRuler DNA Ladder Mix; CK-: Non-transgenic plants; CK+: cDNA of A. majus with mixture two primers AS1 and 4’CGT; (1): without injection; (2): injection without gene construct; (3): injection with gene constructs 4’CGT+AS1