Table 1 Summary and classification of the articles by using non-scaffold to induce odontoblast
From: Mesenchymal stem cells: A comprehensive methods for odontoblastic induction
No | Author | Year | Cell type | Odontoblastic induction methods | Result | Conclusion |
|---|---|---|---|---|---|---|
1 | Hyun Jung Oh et al | 2015 | - MDPC-23 - ALCs - DPCs - C3H10T1/2 - HEK293 | - Transfection of MDPC-23 cells with constructs encoding Cpne7 and Cpne7 siRNA | - Recombinant CPNE7 treated DPCs culture increased expression of DSPP mRNA and DSPP - Mineralized nodule formation was enhanced, and dentin/pulp-like tissue formation observed in DPCs | Cpne7 regulates odontoblast differentiation and dentin formation in vitro |
2 | Naoki Umemura et al | 2016 | - hDPSCs | - Analysis of CD44 expression of DPSC and effects of hyaluronan on the cell cycle - Measurement of BMP-2, BMP-4, DSPP and DMP-1 levels - Examination of DPSC cell signalling | - Number of CD44-expressing cells increased following treatment with HA - ALP proteins level increased in a concentration-dependent manner - Compared to control, the expression of DMP-1 and DSPP mRNA level increased in 7.7-fold and 6.7fold respectively in HA treated cells | - HA induces odontoblastic differentiation in DPSCs via CD44, but does not promote cell proliferation |
3 | Zhuo Chen et al | 2016 | - iMDP-3 | - iMDP23 were induced with odontogenic medium - cells were transfected with Klf10 expression vector | - Klf10 upregulated the expression of DMP-11, DSPP and RUNX2 in iMDP-3 differentiation | - Klf10 promotes odontoblastic differentiation via the up-regulation of DMP1 and DSPP transcription |
4 | Jihua Chai et al | 2019 | - hDPSCs from third molar - Blood samples from healthy patients | - Effects of liquid PRF and PRP were assessed for cellular migration, proliferation and odontoblastic differentiation | - Liquid PRF treated cells showed significant increase in migration and greater ALP activity - When liquid PRF treated cells was cultured within inflammatory environment, the reduced regenerative potential was improved, facilitating hDPC regeneration | - Liquid PRF attenuated the inflammatory condition created by LPS and maintained a supportive regenerative ability for the stimulation of odontoblastic differentiation and reparative dentin in hDPCs |
5 | Lu Yan et al | 2017 | - Human healthy premolars and third molars | - The cells were cultured in odontoblastic induction medium containing various concentrations of high glucose | - Exposure to high glucose (25 and 50 mM) inhibited odontoblastic mineralization and reduced ALP activities - IGF-1 significantly reversed the effects of high glucose by restoring ALP activity and mineralization of DPCs | - IGF-1 restores the reduction of ALP activity and mineralization induced by high glucose. This indicates that IGF-1 attenuates the high glucose-induced inhibition of DPC proliferation, differentiation and mineralization |