Fig. 4

Effects of rapamycin administration on retinal degeneration. (a) Changes in the thickness of the retinal outer nuclear layer (ONL) were assessed continuously by hematoxylin–eosin staining at the ages of 9 to 12 days, after intravitreal administration of rapamycin at the age of 8 days. Wild-type rat retinas are shown in panels i, iv, vii, and x. Compared to the vehicle group, the loss of the ONL was suppressed in the rapamycin group (ii versus iii; v versus vi; viii versus ix; xi versus xii). Scale bar: 30 μm; vertical bar: ONL. (b) Graph showing the ONL thickness in P347L rats with or without rapamycin. Statistical analysis was performed by two-way ANOVA with Sidak’s multiple-comparisons test: ****p < 0.0001; ***p < 0.001; **p < 0.01