Fig. 1

Retinal degeneration in P347L rats. (a) The retina was analyzed in P347L rats from the ages of 9 to 14 days by hematoxylin–eosin staining. In wild-type rats, the outer nuclear layer (ONL) was fully formed at the age of 9 days (i), and the inner and outer segments expanded up to the age of 14 days (xi). In P347L rats, the ONL decreased from the age of 9 days (ii), and this layer was no longer discernible at 14 days (xii). Heterotopic cells were found in the inner segment in the ONL of P347L rats. Retinas were examined at the age of 9 days (i, ii), 10 days (iii, iv); 11 days (v, vi); 12 days (vii, viii); 13 days (ix, x); and 14 days (xi, xii). Arrowheads: heterotopic cells; scale bar: 30 μm; vertical bar: ONL. (b) Graph showing the outer nuclear thickness. Statistical analysis was performed by two-way ANOVA with Sidak’s multiple comparisons test: **p < 0.01; ****p < 0.0001