Table 1 Primer sets used for PCR analysis
Target gene | Primer sequence (5’-3’) | Amplicon size (bp) | Sequence ID |
|---|---|---|---|
Used for first PCR | |||
ALP | Forward: TGG CCC CCA TGC TGA GTG ACA C Reverse: TGG CGC AGG GGC ACA GCA GAC | 160 | NM_000478.4 |
OCT3/4 | Forward: ATT TCA CCA GGC CCC CGG CT Reverse: GCT GAT CTG CTG CAG TGT GGG | 434 | NM_002701.4 |
SOX2 | Forward: TAA ATA CCG GCC CCG GCG GA Reverse: AGG TCC ATT CCC CCG CCC TC | 131 | NM_003106.2 |
GAPDH | Forward: TCC AAA ATC AAG TGG GGC GAT GCT GGC GCT GAG TAC Reverse: GTT GTC ATA CTT CTC ATG GTT CAC ACC CAT GAC GAA | 180 | NM_002046 |
Used for nested PCR | |||
ALP | Forward: CTA AGT GAC ACA GAC AAG AAG Reverse: GGC ACA GCA GAC TGC GCC TGG | 140 | NM_000478.4 |
OCT3/4 | Forward: GGG ACA CCT GGC TTC GGA TTT CGC Reverse: TCT TTC TGC AGA GCT TTG ATG TCC TG | 428 | NM_002701.4 |
SOX2 | Forward: AGC TAC AGC ATG ATG CAG GAC CAG C Reverse: GTC ATG GAG TTG TAC TGC AGG GCG | 125 | NM_003106.2 |
GAPDH | Forward: GTC GTG GAG TCC ACT GGC GTC Reverse: ACA TGG GGG CAT CAG CAG AGG | 109 | NM_002046 |