Fig. 6
From: Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris
Hydrolytic activity assay of ocriplasmin variants measured at 405 nm. The product formation (color intensity) of mutant variants presented much rapid reduction, compared to the wild-type. Data indicate mean of duplicate measurements and analyzed by one-way ANOVA test for detecting significant differences compared to wild-type (P < 0.01). The bar graphs represent standard deviation of three independent assays that each well was assayed in triplicate